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PLoS One . The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic

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  • PLoS One . The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic


    PLoS One


    . 2021 Jul 20;16(7):e0254486.
    doi: 10.1371/journal.pone.0254486. eCollection 2021.
    The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic


    Pang-Yen Chen 1 2 3 , Cheng-Hao Ko 4 5 , C Jason Wang 6 , Chien-Wei Chen 7 8 9 , Wei-Huai Chiu 5 , Chitsung Hong 4 , Hao-Min Cheng 3 10 11 12 , I-Jen Wang 2 13 14 15 16



    Affiliations

    Abstract

    The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body's immune response from natural COVID-19 illness or vaccines' effects is urgently needed. In the present study, an attempt was made to integrate a newly designed spectrometer to the COVID-19 test strip procedure; this augmentation provides the quantitative capacity to a lateral flow immunoassay (LFIA). Optical interpretation of results by quantitative α index, rather than visual qualification, can be done quickly, in 5-10 minutes. The developed product was compared with several other serological IgM/IgG antibody reagents on the market by recruiting 111 participants suspected of having COVID-19 infection from March to May 2020 in a hospital. Taking RT-PCR as the diagnostic gold standard, the quantitative spectral LIFA platform could correctly detect all 12 COVID-19 patients. Concerning RT-PCR negative patients, all three antibody testing methods found positive cases. The optical-based platform exhibited the ability of early detection of immunoglobulins of RT-PCR negative patients. There was an apparent trend that elevation of IgM levels in the acute phase of infection; then IgG levels rose later. It exhibited the risk of a false-negative diagnosis of RT-PCR in COVID-19 testing. The significant detection ability of this new optical-based platform demonstrated clinical potential.


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