J Med Virol Amplification of Human β-Glucoronidase Gene for Appraising the Accuracy of Negative SARS-CoV-2 RT-PCR Results in Upper Respiratory Tract Specimens

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J Med Virol


. 2020 Jun 2.
doi: 10.1002/jmv.26112. Online ahead of print.
Amplification of Human β-Glucoronidase Gene for Appraising the Accuracy of Negative SARS-CoV-2 RT-PCR Results in Upper Respiratory Tract Specimens


Eliseo Albert ¹ , Blanca Ferrer ² , Ignacio Torres ¹ , Alicia Serrano ² , Mar?a Jes?s Alcaraz ¹ , Javier Buesa ^{1 3} , Carlos Solano ^{2 4} , Javier Colomina ¹ , Felipe Bueno ¹ , Dixie Huntley ¹ , Beatriz Olea ¹ , Arantxa Valdivia ¹ , David Navarro ^{1 3}



Affiliations

• PMID: 32484950
• DOI: 10.1002/jmv.26112

Abstract

Real-time reverse transcription polymerase-chain reaction (RT-PCR) is the mainstay of Covid-19 diagnosis.¹ Up to 30% of patients clinically suspected of Covid-19 may have initial or repeat RT-PCR negative results prior to positive test conversion, most notably when upper respiratory tract (URT) specimens are processed.^2-7 This article is protected by copyright. All rights reserved.

Keywords: Epidemiology; Pandemics; Pathogenesis; Respiratory tract; SARS coronavirus; Virus classification.