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Emerg Infect Dis. 2020 Apr 15;26(7). doi: 10.3201/eid2607.201285. [Epub ahead of print]
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Waggoner JJ, Stittleburg V, Pond R, Saklawi Y, Sahoo MK, Babiker A, Hussaini L, Kraft CS, Pinsky BA, Anderson EJ, Rouphael N.
Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
KEYWORDS:
2019 novel coronavirus disease; COVID-19; SARS; SARS-CoV-2; coronavirus; coronavirus disease; molecular detection; rRT-PCR; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses
PMID:32294051DOI:10.3201/eid2607.201285
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