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J Respir Biol Transl Med . Single Cell Analysis of Lung Lymphatic Endothelial Cells and Lymphatic Responses during Influenza Infection

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  • J Respir Biol Transl Med . Single Cell Analysis of Lung Lymphatic Endothelial Cells and Lymphatic Responses during Influenza Infection

    J Respir Biol Transl Med


    . 2024 Mar;1(1):10003.
    doi: 10.35534/jrbtm.2024.10003. Epub 2024 Feb 19. Single Cell Analysis of Lung Lymphatic Endothelial Cells and Lymphatic Responses during Influenza Infection

    Jian Ge 1 , Hongxia Shao 1 2 , Hongxu Ding 3 , Yuefeng Huang 4 , Xuebing Wu 5 , Jie Sun 6 , Jianwen Que 1



    AffiliationsAbstract

    Tissue lymphatic vessels network plays critical roles in immune surveillance and tissue homeostasis in response to pathogen invasion, but how lymphatic system per se is remolded during infection is less understood. Here, we observed that influenza infection induces a significant increase of lymphatic vessel numbers in the lung, accompanied with extensive proliferation of lymphatic endothelial cells (LECs). Single-cell RNA sequencing illustrated the heterogeneity of LECs, identifying a novel PD-L1+ subpopulation that is present during viral infection but not at steady state. Specific deletion of Pd-l1 in LECs elevated the expansion of lymphatic vessel numbers during viral infection. Together these findings elucidate a dramatic expansion of lung lymphatic network in response to viral infection, and reveal a PD-L1+ LEC subpopulation that potentially modulates lymphatic vessel remolding.

    Keywords: Influenza infection; Lymphatic endothelial cells (LECs); PD-L1; Regeneration; Single cell; lung injury; scRNA-seq.

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