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  • protection of several old H1-vaccines in mice

    http://www.ncbi.nlm.nih.gov/pmc/arti...9/?tool=pubmed

    Protection of Mice against Lethal Challenge with 2009 H1N1 Influenza A Virus
    by 1918-Like and Classical Swine H1N1 Based Vaccines

    14 pages in the .pdf : http://www.ncbi.nlm.nih.gov/pmc/arti...at.1000745.pdf

    Manicassamy et.al




    NL/602 is more lethal to mice than CA/07
    I'm interested in expert panflu damage estimates
    my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

  • #2
    Re: protection of several old H1-vaccines in mice

    11 vaccines examined (only HA ?) <1950 better
    escape mutants map to site sa

    === page 2: 9:41

    H1N1 viruses used in this study are as follows:

    A/Swine/Iowa/30 (Sw/30)
    A/Puerto Rico/8/34 -MSSM (PR8)
    A/Weiss/43 (Wei/43)
    A/New Jersey/8/1976 (NJ/76)
    A/USSR/92/77 (USSR/77)
    A/Houston/20593/84 (Hou/84)
    A/Texas/36/1991 (Tx/91)
    A/Brisbane/59/2007 (Bris/59/07)
    A/California/04/2009 (Cal/09)
    A/Netherlands/602/2009 (Neth/09)

    A/Northern Territory/60/1968 (NT/68)
    A/Brisbane/10/2007 (Bris/10/07)
    as H3N2 controls

    Cal09 6:2 : HA and CA from Cal/09 , rest from PR8

    6B9 : 1918, IgG2a
    39E4 : 1918 , IgG2a
    29E3 : Cal/09 , IgG2b

    === page 3 , 9:53

    2*15myg of inactivated virus or 1918 VLP 14 days apart "vaccination" (=active immunization ?)

    150 myg monoclonal antibodies(mAbs) 6B9,39E4 - , 29E3 "passive immunization"

    Cal/09 : markedly , 5e4pfu, 22.5&#37; weight loss ("wl") by day 8
    Bris/59/07 : fewer
    Neth/09 , 5e4pfu,C57B/6, more wl than with Cal/09
    Cal/09,Neth/09(21d,1e3pfu) all survived
    Cal/09,Neth/09(21d,5e4pfu) few wl

    === page 4 , 10:17
    brb 7min
    figure 1
    what's "mock" "in contrast, mice previously mock-infected succumbed ...")
    mock = placebo ?

    no vax,Neth/09 : "endpoint" by day5
    Cal/09 6:2,Neth/09 : no deaths, modest wl,no virus in lungs
    SW/30,Neth/09 : no deaths , no virus in lungs on day 6, wl=18.2%
    NJ/76,Neth/09 : no deaths , no virus in lungs on day 6 , wl=18.2%
    Wei/4,Neth/09 : no deaths , high virus in lungs on day 6 , wl >18.2%
    1918 VLP,Neth/09 : no deaths, wl similar to {Cal/09 6:2,Neth/09}, no virus in lungs on day 6
    USSR/77,Neth/09 : ~40% deaths, extensive wl, titers on day 6 lower as no vax,Neth/09
    Hou/84,Neth/09 : similar
    Tx/91,Neth/09 : similar
    Bris/59/07,Neth/09 : similar

    === page 5 , 10:55
    graphs ...
    wl and survival strongly depends on initial dose (limit ~5e4pfu)
    NT/68 protects a bit, reason unclear
    "inactivated vaccines" , I still don't know whether this is whole-virus or only HA or HA+NA
    table1, #mouse titers induced by vaccination

    cross reactive neutralizing antibodies , I don't fully understand this , skip that part
    reason for protection are the epitopes (--> humoral ?)

    150mAbs -24h,
    === page 6 , 11:13
    graphs...
    ahh, why do we need text, when we have graphs

    A:wl by day 5 from Neth/09 infection,C57B/6,2*15myg,(14d,14),8e5pfu {do we get that much by breathing ?}


    Code:
    wl by day 5 in %
    % survival by day 5
    10*(log(titers in lungs by day 3))
    10*(log(titers in lungs by day 6))
    10*log(titers from mAbs showing expected protection from CA/09 challenge)
    ----------------------------------------------------------------------------
    04 99 -- --       mock
    22 20 65 46 07    USSR/77
    22 25 62 36 07     NT/68
    22 -- 61 38 07     Hou/84
    22 60 60 16 07     Tx/91
    22 40 63 40 07     Bris/59/07
    24 00 -- --     Bris/10/07
    12 99 -- -- 34     Cal/09 6:2
    12 99 48 -- 22    1918 VLP
    17 99 44 -- 19     Sw/30
    17 99 50 -- 16      NJ/76
    21 99 65 41 07     Wei/43
    27 00 69 55 07    no vax(control)
    -- -- -- -- 28    Neth/09
    27 00 66 59 07    control mAb
    -2 99 38 47 24    29E3(Cal/09)
    01 99 52 51 --    6B9(1918)
    06 99 43 56 21    39E4(1918)
    13 99 49 31 --     Poly(Cal/09)
    -- -- -- -- 16  Cal/09 virus
    {I think, this table will be central in my -hopefully better- summary}

    brb 6min

    specific : 110 titers
    polyclonal: 40 titers

    results from antibody treatment is hopefully shown later in a similar table, so it can
    just be added here. So I skip this part

    === page 7 , 12:22
    table 2
    I never know in these tables what's horizontal and what vertical
    I want protection against potential Cal/09 infection and I take row 10 to measure this
    and include it in my above table

    escape mutants CA/09 6:2
    mAbs 6B9,39E4, (1918-specific) G172E,K171E,
    29E3(******-specific) K171E,K171Q,K180N
    brb 5min

    table 3 HI activity of mAbs {without escape mutants ?}

    Discussion:
    ----------------
    starts as "abstract" or "introduction"
    table 4: in vivo titers
    (integrate in above table)

    Code:
    activity against Cal/09 6:2  (*100 minimal myg/ml)
    activity against Bris/59/07 (*100 minimal myg/ml)
    activity against 1918VLP (*100 minimal myg/ml)
    10*log(HI-titers in vivo)
    name of antibody
    ---------------------------------------------
    15 99 15 --     1918HA  6B9
    15 99 15 21    1918HA 39E4
    04 99 08 24     Cal/09 HA  29E3
    99 99 -- 07     Nipah W ----  
    -- -- -- 16        Cal/09 virus
    === page 8 , 13:08

    figure 4 (include in above table)

    inactivated vaccines induce humoral and minimal cellular immunity

    they think that their findinds for mice will be similar in humans

    === page 9 , 13:27

    nucleotide differences CA/09 -- Neth/09 :
    CA/09: A1218G(1),T1872C(1),G1758A(2),T670C(3),G1986T(3),
    T298C(4),C622A(4),G640A(4),G891A(4),A972G(4),G1012 A(4),A1044G(6)
    Neth/09:T2023C(1),A1741C(3),C1118T(5),A322G(6),A408G(6) ,A945C(6),G1219A(6)

    amino acid differences CA/09 -- Neth/09 :
    CA/09: S224P(3),S100P(4),L208I(4),A214T(4),V338I(4)
    Neth/09: M581L(3),T373I(5),I108V(6),V407I(6)

    figure 5, mutations of the involved viruses at the antigenic sites in HA

    List: (later...)
    Cb(78-83)
    Sa(128-129,156-160,162-167)
    Sb(187-198)
    Ca1(169-173,206-208,238-240
    Ca2(140-145,224-226)
    H3-numbering , Brownlee+Fodor (2001)

    === page 10 , 13:43

    3d-picture of HAs
    however the yellow escape mutation positions are identical in 1918 and 2009

    === page 11 , 13:47

    make a more complete table of antigenic sites of ~50 viruses , human and swine, 1918-2009 (later)
    thinks protection from cell-based immunity and NA play a minor rule
    good protection by Tx/91 is unexplained (I remember that that particular strain caused
    some GB)
    what's monoclonal,polyclonal ?

    1976 - why do we have no estimates, how many % of ******-infected people had had
    the 1976 vax ? (just ask them !)

    the protection back to 1943, does it also hold for the 1977-semi-pandemic , which also
    mainly infected children ?


    so longterm protection (1943 back to 1918 up to 2009 = 116 years
    yet the seasonal vaccine is often a bad match (why ?)

    H1N1 antigenically frozen in pigs ? -----edit----- yes, see e.g. the antigenic site Sb in the table below

    === page 12 , 14:08

    possible future pandemics from frozen viruses {but we can prepare with vaccine}

    same study in humans ? ******=CA/09 is not lethal, usually mild, most people will be infected
    earlier or later anyway. In USA, where this site is located, they even have death-penalty,
    so you could use these people. However, not in the same country since else the judges might
    be more willing to speak death penalties. It all depends on _how many_ such experiments
    in humans you wanted to do. Probably not reasonable in this case, but when we get
    a severe H5N1-pandemic it could be important.
    First verify some few of the results in ferrets or swine


    brb 3min

    === page 13 , 14:23

    references not read


    ~4.5 hours in total , 2 tables still to be made , the central table still to be studied and
    the results comprehended,learned,visualized


    ---------------------------------------------------------------------------------------

    the tables are not near the referring text

    the whole paper writing process is ineffective, you need to long to rea it and the abstract
    and author summary is not informative enough (IMO)
    Why these long papers and no shorter presentations ?
    In math you skip the proofs and just read the theorems for a first understanding,
    what are the theorems here ?

    it took 9 months, too late for wave 1,wave 2,(wave 3 in Mex.)

    pdf , no data are given , so I had to estimate the values for my central table
    from graphs.
    Data would be useful so we can make our own tables, own graphs ,
    maybe better presented. I assume this is not in the interest of the authors and that's
    why data and computer-readable tables and plain text (easy to quote and discuss in forums)
    are usually not provided (?)
    I'm interested in expert panflu damage estimates
    my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

    Comment


    • #3
      Re: protection of several old H1-vaccines in mice

      Code:
      antigenic sites of early human H1
       Cb          Sa               Sb           Ca1           Ca2
      -----------xx--------x------------------------------------------------------------------
      SWSYIV  NH KKGSS PKLSKS  TGTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RDQ  >A/Brevig Mission/1/1918
      SWSYIV  NH KKGSS PKLSKS  TGTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RGQ  >A/New_York/1/18
      SWSYIV  NH KKGSS PKLSKS  TGTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RDQ  >A/South Carolina/1/1918
      SWSYIV  NH KKGSS PKLSKS  TSTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RDQ  >A/London/1/1918
      SWSYIV  NH KKGSS PKLSKS  TGTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RGQ  >A/London/1/1919
      SWSYIV  NH KKGDS PKLTNS  SSDEQQSLYSNG  VNNKG SSN EPG  SHRGKS KDQ  >A/WSN/1933
      SWSYIV  NH KXGDS PKLNNS  SSNEQQSLYHNV  VNNKG SSN EPG  SHGGKS RDQ  >A/Wilson-Smith/1933
      SWSYIV  NH KKGDS PKLTNS  SSDEQQSLYSNG  VNNKG SSN EPG  SHRGKS RDQ  >A/Wilson-Smith/1933
      SWSYIV  NH KKGDS PKLTNS  SSDEQQSLYSNG  VNNKG SSN EPG  SHRGKS KDQ  >A/WSN/1933 TS61
      SWSYIV  NH KKGDS PKLTNS  SSDEQQSLYSNG  VNNKG SSN EPG  SHRGKS KDQ  >A/WSN/1933
      SWSYIV  NH KTGDS PKLNNS  SSNEQQSLYHNV  VNNKG SSN EPG  SHGGKS RDQ  >A/United Kingdom/1/1933,33/04/16
      SWSYIV  NH EKEGS PKLKNS  NSKDQQNIYQNE  VNKKG TSN KPG  SHAGKS RDQ  >A/PR/8/34(H0N1)
      SWSYIV  NH EKEGS PKLKNS  NSKEQQNLYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Puerto Rico/8/34
      SWSYIV  NH EKEGS PKLKNS  ------------  VN--- --- ---  SHEGKS ---  >A/Puerto Rico/8/34
      SWSYIV  NH EKEGS PKLKNS  NSKDQQNIYQNE  VNKKG TSN KPG  SHAGKS RDQ  >A/Puerto Rico/8/34
      SWSYIV  NH EKEGS PKLKNS  NSKDQQNIYQNE  VNKKG TSN KPG  SHAGKS RDQ  >A/Puerto Rico/8/34/PY102-V1
      SWSYIV  NH EKEGS PKLKNS  NSKDQQNIYQNE  ENKKG TSN KPG  SHAGKS RDQ  >A/VM113-V1
      SWSYIV  NH EKEGS PKLKNS  NSKEQQNIYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Puerto Rico/8/34/Mount Sinai
      SWSYIV  NH EKEGS PKLKNS  NSKEQQNLYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Puerto Rico/8/34
      SWSYIV  NH EKEGS PKLKNS  NSKEQQNLYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Puerto Rico/8/34
      SWSYIV  NH EKEGS PNLKNS  NSKEQQNLYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Puerto Rico/8/34
      SWSYIV  DH KKESS PELKNS  DSEEQQNLYQNE  VNKKR TSN KPG  SHEGKN KGQ  >A/Puerto Rico/8-SV14/1934
      SWSYIV  NH EKEGS PKLKNS  NSKEQQNLYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Puerto Rico/8-1/1934
      SWSYIV  KH KKEDS PKLSNS  NSKEQQTLYQNE  VNKKG SSN EPG  SHAGKS RDQ  >A/Melbourne/1935
      SWSYIV  NH EKEGS PKLKNS  NSKEQQNLYQNE  VNKKG TSN KPG  SHEGKS RDQ  >A/Alaska/1935
      SWSYIV  KH KKEDS PKLKNS  SSKEQQTLYQNE  VNKKG SSN EPG  SHAGKS RDQ  >A/Phila/1935
      SWSYIV  NH KKGGS PKLKNS  NSKDQQTLYQNE  VNKKG SSN EPG  SHAGKS RDQ  >A/Henry/1936
      SWSYIA  NH EKDGS PNLNKS  NIENQKTLYRKE  VNKKE SSN EPG  SHAGKS RGQ  >A/Hickox/1940
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN EPG  SHAGKC RGQ  >A/Bellamy/1942
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN KPG  SHAGKS RGQ  >A/DSP/43
      SWSYIV  KH EKDGS PYLRNS  SIKEQQTLYQKE  VNKKA CSN EPG  SHAGKR RDQ  >A/Weiss/1943
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN KPG  SHAGKS RGQ  >A/Marton/43
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN EPG  SHAGKS RGQ  >A/Huston/43
      SWSYIV  KH EKDGS PNLKNS  SIKEQQTLYQKE  VNKKG SSN EPG  SHAGKS RDQ  >A/Weiss/1943
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN KPG  SHAGKS RGQ  >A/AA/Marton/1943
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN KPG  SHAGKS RGQ  >A/Iowa/1943
      SWSYIV  KH EKDGS PNLNNS  NIKDQQTLYQKE  VNKKG SSN KPG  SHAGKS RGQ  >A/AA/Huston/1945
      SWSYIA  EH EKDGS PNLNKS  NIENQKTLYRKE  VNKKE SSN EPG  SHAGKS RGQ  >A/Cameron/1946
      SWSYIV  KH KKEDS PKLSNS  SSKEQQTLYQNE  VNKKG SSN EPG  SHAGKS KDQ  >A/Melbourne/1/1946
      SWSYIA  KH ETDGS PKLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHAGKS RGQ  >A/Fort Monmouth/1/1947
      SWSYIA  KH ETDGS PKLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHAGKS RGQ  >A/Fort Monmouth/1/1947
      SWSYIA  KH ETDGS PNLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHAGKS RGQ  >A/Rhodes/47
      SWSYIA  KH ETDGS PKLSKS  NIEDQKTLYRKE  VNNNE SSN EPG  SHAGKS RGQ  >A/Fort Monmouth/1/1947
      SWSYIA  KH ETNGS PKLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHAGKS RGQ  >A/Fort Monmouth/1/1947
      SWSYIA  KH ETDGS PKLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHAGKS RGQ  >A/Fort Monmouth/1/1947
      SWSYIA  KH EKNGS PTLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Lepine/1948
      SWSYIA  KH EKNGS PNLNKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Albany/4835/1948
      SWSYIA  KH EKDGS PNLSKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Roma/1949
      SWSYIA  KH EANGS PALSTS  NIEEQRTLYRKD  VNNQE SSN EPG  SHAGKS RDQ  >A/Fort Worth/1950
      SWSYIA  KH EKNGS PNLKKS  NIEDQKTLYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Albany/4836/1950
      SWSYIA  KH EANGS PNLSES  NIEDQRTLYRKE  VNNKE SSN EPG  SHAKKS RGQ  >A/TF/15/1951
      SWSYIA  KH EANGS PNLSKS  NIEDQRTLYRKE  VNNKE SSN EPG  SHAKKS RGQ  >A/Tokyo/1/51
      SWSYIA  KH EANGS PNLSKS  NIEDQRTLYRKE  VNNKE SSN EPG  SHAKKS RGQ  >A/Albany/12/1951
      SWSYIA  KH EANGS PNLSKS  KIEDQRTLYRKE  VNNKE SSN EPG  SHAKKS RGQ  >A/Albany/1618/1951
      SWSYIA  KH EANGS PNLSKS  NIEDQRTLYRKE  VNNKE SSN EPG  SHAKKS RGQ  >A/Albany/14/1951
      SWSYIA  KH EANGS PNLSKS  NIEDQRTLYRKE  VNNKE SSN EPG  SHAKKS RGQ  >A/Albany/13/1951
      SWSYIA  KH EANGS PNLSKS  NIEDQRTLYRKD  VNNQE SSN EPG  SHAGKS RGQ  >A/Kojiya/1/1952
      SWSYIA  KH EKNGS PNLSKS  NIEDQKTIYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Leningrad/1954/1
      SWSYIA  NH EANGS PNLSRS  NIEEQRTLYRKD  VNNQE SSN EPG  SHARKS RDQ  >A/Taiwan/13/1954
      SWSYIA  KH EANGL PSLSKS  NIEDQRTLYRKE  VNDRE SSN EPG  SHAKKS RGQ  >A/Malaysia/1954
      SWSYIA  KH EANGL PSLSKS  NIEDQRTLYRKE  VNDRE SSN EPG  SHAKKS RGQ  >A/Malaya/302/1954
      SWSYIA  KH EANGS PNLSNS  NIEEQRTLYRKD  VNNQE SSN EPG  SHARKS RGQ  >A/Yamagishi/55
      SWSYIA  KH EANGS PTLSNS  NIEEQRTLYRKD  VNNQE SSN EPG  SHARKS RDQ  >A/Meguro/1/1956
      SWSYIA  NH EANGS PNLSRS  NIEEQRTLYRKD  VNNQE SSN EPG  PHAKKS RGQ  >A/Saga/2/1957
      SWSYIA  NH EANGS PNLSRS  NIEEQRALYRKD  VNNQE SSN EPG  PHARKS RDQ  >A/Denver/1957
      
      SWSYIV  NH KKGNS PKLSKS  TSADQQSLYQNA  INDKG SSR EPG  PHAGAK RDQ  >A/******/index/2009/02/01
      SWSYIV  NH KKENS PKLSKS  TSTDQQSLYQNA  VNNKG SSK EPG  PYAGAN RGQ  >A/New Jersey/1976
      SWSYIV  NH KKENS PKLSKS  TSTDQQSLYQNA  VNNKG SSK EPG  PYAGAS RGQ  >A/swine/Iowa/15/1930
      SWSYIV  NH KKGSS PKLSKS  TGTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RDQ  >A/Brevig Mission/1/1918
      SWSYIV  KH EKDGS PNLKNS  SIKEQQTLYQKE  VNKKG SSN EPG  SHAGKS RDQ  >A/Weiss/1943
      SWSYIA  KH EKNGS PNLSKS  NIEDQKTIYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Index-Russian flu/1977
      SWSYIA  NH KKNGL PNLSKS  NIGDQRAIYHTE  VNNKE SSH EPG  SHNGES RGQ  >A/Texas/36/1991
      SWSYIV  NH GKNGL PNLSKS  NIGIQKALYHTE  ANNKE SSH EPG  SHNGES RDQ  >A/Brisbane/59/2007/07/01

      Code:
      SWSYIV  NH SFYKNLIWLVKKGNSYPKLSKS  TSADQQSLYQNA  INDKG SSR EPG  PHAGAK RDQ  >A/******/index/2009/02/01
      SWSYIV  NH SFYRNLIWLVKKENSYPKLSKS  TSTDQQSLYQNA  VNNKG SSK EPG  PYAGAN RGQ  >A/New Jersey/1976
      SWSYIV  NH SFYRNLLWLVKKENSYPKLSKS  TSTDQQSLYQNA  VNNKG SSK EPG  PYAGAS RGQ  >A/swine/Iowa/15/1930
      SWSYIV  NH SFYRNLLWLTKKGSSYPKLSKS  TGTDQQSLYQNA  VNNKG SSK EPG  SYAGAS RDQ  >A/Brevig Mission/1/1918
      SWSYIV  KH SFYRNLLWLTEKDGSYPNLKNS  SIKEQQTLYQKE  VNKKG SSN EPG  SHAGKS RDQ  >A/Weiss/1943
      SWSYIA  KH SFYRNLLWLTEKNGSYPNLSKS  NIEDQKTIYRKE  VNNKE SSN EPG  SHKGKS RGQ  >A/Index-Russian flu/1977
      SWSYIA  NH SFYRNLLWLTKKNGLYPNLSKS  NIGDQRAIYHTE  VNNKE SSH EPG  SHNGES RGQ  >A/Texas/36/1991
      SWSYIV  NH SFYRNLLWLTGKNGLYPNLSKS  NIGIQKALYHTE  ANNKE SSH EPG  SHNGES RDQ  >A/Brisbane/59/2007/07/01


      please try the mutant N177K of the Brisbane vax
      I'm interested in expert panflu damage estimates
      my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

      Comment


      • #4
        Re: protection of several old H1-vaccines in mice

        Strains of influenza A virus show marked differences in their
        ability to infect murine macrophages
        http://www.ncbi.nlm.nih.gov/pubmed/20142836
        I'm interested in expert panflu damage estimates
        my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

        Comment


        • #5
          Re: protection of several old H1-vaccines in mice

          ahh, these K171Q,K171E,K180N were "escape mutants", apparantly
          happened after lots of passages and lots of vaccinations
          to "escape" the vaccine

          The escape mutants that arose in the presence of the 1918 specific mAb 6B9 and 39E4
          contained mutations G172E (G158E by H3 numbering) and K171E (K157E by H3 numbering), respectively. The escape mutants generated by selection with the 2009 H1N1 specific mAb (29E3) carried either a K171E or K171Q or K180N mutation (residues K171 and K180 correspond to K157 and K166 in H3 numbering). These results indicate that all the mAbs bind to the conserved antigenic site Sa (Fig. 5 and 6).
          I'm interested in expert panflu damage estimates
          my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

          Comment


          • #6
            Re: protection of several old H1-vaccines in mice

            1)age,
            % of people with titers >1:40 against:
            2)CA/2007
            3)Brisbane/2007
            4)Denver/1957
            5)SC/1918
            Code:
             1)   2) 3) 4) 5)
            -------------------------
            00-09 28 47 36 02
            10-19 45 50 44 05
            20-29 20 39 54 13
            30-39 14 18 17 10
            40-49 18 19 37 14
            50-59 22 20 58 11
            60-69 13 21 45 13
            70-79 05 15 50 48
            80-89 26 11 22 59
            pre-- 06 22 33 01
            I'm interested in expert panflu damage estimates
            my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

            Comment

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