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Blood. 2019 Feb 5. pii: blood-2018-09-873984. doi: 10.1182/blood-2018-09-873984. [Epub ahead of print]
Human megakaryocytes possess intrinsic anti-viral immunity through regulated induction of IFITM3.
Campbell RA1, Schwertz H2, Hottz ED3, Rowley JW1, Manne BK1, Washington AV4, Hunter-Mellado R5, Tolley ND1, Christensen M6, Eustes AS1, Montenont E1, Bhatlekar S1, Ventrone CH7, Kirkpatrick BD7, Pierce KK7, Whitehead SS8, Diehl SA7, Bray PF1, Zimmerman GA1, Kosaka Y1, Bozza PT3, Bozza FA3, Weyrich AS1, Rondina MT9.
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Abstract
Evolving evidence indicates that platelets and megakaryocytes (MKs) have unexpected activities in inflammation and infection; but whether viral infections upregulate biologically active, anti-viral immune genes in platelets and MKs is unknown. We examined anti-viral immune genes in these cells in dengue and influenza infections, viruses that are global public health threats. Using complementary biochemical, pharmacological, and genetic approaches, we examined the regulation and function of interferon induced transmembrane protein 3 (IFITM3), an anti-viral immune effector gene not previously studied in human platelets and MKs. IFITM3 was markedly upregulated in platelets isolated from patients during clinical influenza and dengue (DENV) infections. Lower IFITM3 expression in platelets correlated with increased illness severity and mortality in patients. Administering a live, attenuated DENV vaccine to healthy subjects significantly increased platelet IFITM3 expression. Infecting human MKs with DENV selectively increased type I interferons (IFNs) and IFITM3. Overexpression of IFITM3 in MKs was sufficient to prevent DENV infection. In naturally-occurring, genetic loss-of-function studies MKs from healthy subjects harboring a homozygous mutation in IFITM3 (rs12252-C, a common SNP in areas of the world where DENV is endemic) were significantly more susceptible to DENV infection. DENV-induced MK secretion of IFNs prevented infection of bystander MKs and hematopoietic stem cells (HSCs). Thus, viral infections upregulate IFITM3 in human platelets and MKs, and IFITM3 expression is associated with adverse clinical outcomes. These observations establish, for the first time, that human MKs possess anti-viral functions, preventing DENV infection of MKs and HSCs following local immune signaling.
Copyright ? 2019 American Society of Hematology.
PMID: 30723081 DOI: 10.1182/blood-2018-09-873984
Human megakaryocytes possess intrinsic anti-viral immunity through regulated induction of IFITM3.
Campbell RA1, Schwertz H2, Hottz ED3, Rowley JW1, Manne BK1, Washington AV4, Hunter-Mellado R5, Tolley ND1, Christensen M6, Eustes AS1, Montenont E1, Bhatlekar S1, Ventrone CH7, Kirkpatrick BD7, Pierce KK7, Whitehead SS8, Diehl SA7, Bray PF1, Zimmerman GA1, Kosaka Y1, Bozza PT3, Bozza FA3, Weyrich AS1, Rondina MT9.
Author information
Abstract
Evolving evidence indicates that platelets and megakaryocytes (MKs) have unexpected activities in inflammation and infection; but whether viral infections upregulate biologically active, anti-viral immune genes in platelets and MKs is unknown. We examined anti-viral immune genes in these cells in dengue and influenza infections, viruses that are global public health threats. Using complementary biochemical, pharmacological, and genetic approaches, we examined the regulation and function of interferon induced transmembrane protein 3 (IFITM3), an anti-viral immune effector gene not previously studied in human platelets and MKs. IFITM3 was markedly upregulated in platelets isolated from patients during clinical influenza and dengue (DENV) infections. Lower IFITM3 expression in platelets correlated with increased illness severity and mortality in patients. Administering a live, attenuated DENV vaccine to healthy subjects significantly increased platelet IFITM3 expression. Infecting human MKs with DENV selectively increased type I interferons (IFNs) and IFITM3. Overexpression of IFITM3 in MKs was sufficient to prevent DENV infection. In naturally-occurring, genetic loss-of-function studies MKs from healthy subjects harboring a homozygous mutation in IFITM3 (rs12252-C, a common SNP in areas of the world where DENV is endemic) were significantly more susceptible to DENV infection. DENV-induced MK secretion of IFNs prevented infection of bystander MKs and hematopoietic stem cells (HSCs). Thus, viral infections upregulate IFITM3 in human platelets and MKs, and IFITM3 expression is associated with adverse clinical outcomes. These observations establish, for the first time, that human MKs possess anti-viral functions, preventing DENV infection of MKs and HSCs following local immune signaling.
Copyright ? 2019 American Society of Hematology.
PMID: 30723081 DOI: 10.1182/blood-2018-09-873984