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Biochem Biophys Res Commun. 2017 Sep 5. pii: S0006-291X(17)31771-0. doi: 10.1016/j.bbrc.2017.09.011. [Epub ahead of print]
Roles of antibodies to influenza A virus hemagglutinin, neuraminidase, and M2e in conferring cross protection.
Kim YJ1, Ko EJ1, Kim MC1, Lee YN1, Kim KH1, Jung YJ1, Kang SM2.
Author information
Abstract
Although neuraminidase (NA) is the second major viral glycoprotein of influenza virus, its immune mechanism as a vaccine target has been less considered. Here we compared the properties of antibodies and the efficacy of cross protection by N1 and N2 NA proteins, inactivated split influenza vaccines (split), and tandem repeat extracellular domain M2 on virus-like particles (M2e5x VLP). Anti-NA immune sera could confer better cross-protection against multiple heterologous influenza viruses correlating with NA inhibition activity compared to split vaccine immune sera. Whereas split vaccine was superior to NA in conferring homologous protection. NA and M2e immune sera each showed comparable survival protection. Protective efficacy by NA immune sera was lower in Fc receptor common γ-chain deficient mice but comparable in C3 complement deficient mice compared to that in wild type mice, suggesting a role of Fc receptor in NA immunity.
Copyright ? 2017. Published by Elsevier Inc.
KEYWORDS:
Influenza virus; M2e; Neuraminidase; Split vaccines
PMID: 28887040 DOI: 10.1016/j.bbrc.2017.09.011
Roles of antibodies to influenza A virus hemagglutinin, neuraminidase, and M2e in conferring cross protection.
Kim YJ1, Ko EJ1, Kim MC1, Lee YN1, Kim KH1, Jung YJ1, Kang SM2.
Author information
Abstract
Although neuraminidase (NA) is the second major viral glycoprotein of influenza virus, its immune mechanism as a vaccine target has been less considered. Here we compared the properties of antibodies and the efficacy of cross protection by N1 and N2 NA proteins, inactivated split influenza vaccines (split), and tandem repeat extracellular domain M2 on virus-like particles (M2e5x VLP). Anti-NA immune sera could confer better cross-protection against multiple heterologous influenza viruses correlating with NA inhibition activity compared to split vaccine immune sera. Whereas split vaccine was superior to NA in conferring homologous protection. NA and M2e immune sera each showed comparable survival protection. Protective efficacy by NA immune sera was lower in Fc receptor common γ-chain deficient mice but comparable in C3 complement deficient mice compared to that in wild type mice, suggesting a role of Fc receptor in NA immunity.
Copyright ? 2017. Published by Elsevier Inc.
KEYWORDS:
Influenza virus; M2e; Neuraminidase; Split vaccines
PMID: 28887040 DOI: 10.1016/j.bbrc.2017.09.011