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J Infect Dis. 2016 Sep 6. pii: jiw413. [Epub ahead of print]
HUMAN ALVEOLAR MACROPHAGES MAY NOT BE SUSCEPTIBLE TO DIRECT INFECTION BY A HUMAN INFLUENZA VIRUS.
Ettensohn DB1, Frampton MW1, Nichols JE2, Roberts NJ Jr3.
Author information
Abstract
The current studies were undertaken to determine the susceptibility of human alveolar macrophages (AM) to influenza A virus (IAV) infection in comparison with autologous peripheral blood-derived monocytes-macrophages (PBM). AM and PBM were exposed to IAV in vitro and examined for their ability to bind and internalize IAV, and synthesize viral proteins and RNA. PBM but not AM demonstrated binding and internalization of the virus, synthesizing viral proteins and RNA. Exposure of AM in the presence of a sialidase inhibitor, or in the presence of anti-IAV antibody resulted in viral protein synthesis by the cells. Exposure of AM to FITC-labeled IAV in the presence of anti-FITC antibody also resulted in viral protein synthesis. Thus, human AM are apparently not susceptible to direct infection by a human IAV, but are likely to be infected indirectly in the setting of exposure in the presence of antibody that binds the challenging strain of IAV.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PMID: 27601618 DOI: 10.1093/infdis/jiw413
[PubMed - as supplied by publisher]
HUMAN ALVEOLAR MACROPHAGES MAY NOT BE SUSCEPTIBLE TO DIRECT INFECTION BY A HUMAN INFLUENZA VIRUS.
Ettensohn DB1, Frampton MW1, Nichols JE2, Roberts NJ Jr3.
Author information
Abstract
The current studies were undertaken to determine the susceptibility of human alveolar macrophages (AM) to influenza A virus (IAV) infection in comparison with autologous peripheral blood-derived monocytes-macrophages (PBM). AM and PBM were exposed to IAV in vitro and examined for their ability to bind and internalize IAV, and synthesize viral proteins and RNA. PBM but not AM demonstrated binding and internalization of the virus, synthesizing viral proteins and RNA. Exposure of AM in the presence of a sialidase inhibitor, or in the presence of anti-IAV antibody resulted in viral protein synthesis by the cells. Exposure of AM to FITC-labeled IAV in the presence of anti-FITC antibody also resulted in viral protein synthesis. Thus, human AM are apparently not susceptible to direct infection by a human IAV, but are likely to be infected indirectly in the setting of exposure in the presence of antibody that binds the challenging strain of IAV.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PMID: 27601618 DOI: 10.1093/infdis/jiw413
[PubMed - as supplied by publisher]