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EBioMedicine. 2016 Jun;8:277-90. doi: 10.1016/j.ebiom.2016.04.029. Epub 2016 Apr 28.
What Lies Beneath: Antibody Dependent Natural Killer Cell Activation by Antibodies to Internal Influenza Virus Proteins.
Vanderven HA1, Ana-Sosa-Batiz F1, Jegaskanda S1, Rockman S2, Laurie K3, Barr I3, Chen W4, Wines B5, Hogarth PM5, Lambe T6, Gilbert SC6, Parsons MS1, Kent SJ7.
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Abstract
The conserved internal influenza proteins nucleoprotein (NP) and matrix 1 (M1) are well characterised for T cell immunity, but whether they also elicit functional antibodies capable of activating natural killer (NK) cells has not been explored. We studied NP and M1-specific ADCC activity using biochemical, NK cell activation and killing assays with plasma from healthy and influenza-infected subjects. Healthy adults had antibodies to M1 and NP capable of binding dimeric FcγRIIIa and activating NK cells. Natural symptomatic and experimental influenza infections resulted in a rise in antibody dependent NK cell activation post-infection to the hemagglutinin of the infecting strain, but changes in NK cell activation to M1 and NP were variable. Although antibody dependent killing of target cells infected with vaccinia viruses expressing internal influenza proteins was not detected, opsonising antibodies to NP and M1 likely contribute to an antiviral microenvironment by stimulating innate immune cells to secrete cytokines early in infection. We conclude that effector cell activating antibodies to conserved internal influenza proteins are common in healthy and influenza-infected adults. Given the significance of such antibodies in animal models of heterologous influenza infection, the definition of their importance and mechanism of action in human immunity to influenza is essential.
Copyright ? 2016 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:
Antibody dependent cellular cytotoxicity; Hemagglutinin; Influenza; Matrix protein 1; Natural killer cells; Nucleoprotein
PMID: 27428437 DOI: 10.1016/j.ebiom.2016.04.029
[PubMed - in process]
What Lies Beneath: Antibody Dependent Natural Killer Cell Activation by Antibodies to Internal Influenza Virus Proteins.
Vanderven HA1, Ana-Sosa-Batiz F1, Jegaskanda S1, Rockman S2, Laurie K3, Barr I3, Chen W4, Wines B5, Hogarth PM5, Lambe T6, Gilbert SC6, Parsons MS1, Kent SJ7.
Author information
Abstract
The conserved internal influenza proteins nucleoprotein (NP) and matrix 1 (M1) are well characterised for T cell immunity, but whether they also elicit functional antibodies capable of activating natural killer (NK) cells has not been explored. We studied NP and M1-specific ADCC activity using biochemical, NK cell activation and killing assays with plasma from healthy and influenza-infected subjects. Healthy adults had antibodies to M1 and NP capable of binding dimeric FcγRIIIa and activating NK cells. Natural symptomatic and experimental influenza infections resulted in a rise in antibody dependent NK cell activation post-infection to the hemagglutinin of the infecting strain, but changes in NK cell activation to M1 and NP were variable. Although antibody dependent killing of target cells infected with vaccinia viruses expressing internal influenza proteins was not detected, opsonising antibodies to NP and M1 likely contribute to an antiviral microenvironment by stimulating innate immune cells to secrete cytokines early in infection. We conclude that effector cell activating antibodies to conserved internal influenza proteins are common in healthy and influenza-infected adults. Given the significance of such antibodies in animal models of heterologous influenza infection, the definition of their importance and mechanism of action in human immunity to influenza is essential.
Copyright ? 2016 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:
Antibody dependent cellular cytotoxicity; Hemagglutinin; Influenza; Matrix protein 1; Natural killer cells; Nucleoprotein
PMID: 27428437 DOI: 10.1016/j.ebiom.2016.04.029
[PubMed - in process]
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