Int J Infect Dis Aberrant Hyperactivation of Cytotoxic T-cell as a Potential Determinant of COVID-19 Severity

[tetano]

Int J Infect Dis


. 2020 May 31;S1201-9712(20)30413-6.
doi: 10.1016/j.ijid.2020.05.106. Online ahead of print.
Aberrant Hyperactivation of Cytotoxic T-cell as a Potential Determinant of COVID-19 Severity


Chang Kyung Kang ¹ , Gi-Chan Han ² , Minji Kim ² , Gwanghun Kim ² , Hyun Mu Shin ³ , Kyoung-Ho Song ⁴ , Pyoeng Gyun Choe ⁵ , Wan Beom Park ⁵ , Eu Suk Kim ⁶ , Hong Bin Kim ⁶ , Nam-Joong Kim ⁵ , Hang-Rae Kim ⁷ , Myoung-Don Oh ⁸



Affiliations

• PMID: 32492530
• DOI: 10.1016/j.ijid.2020.05.106

Abstract

Objectives: We hypothesized that immune response may contribute to progression of coronavirus disease-19 (COVID-19) at the second week of illness. Therefore, we compared cell-mediated immune (CMI) responses between severe and mild COVID-19 cases.
Methods: We examined peripheral blood mononuclear cells of laboratory-confirmed COVID-19 patients from their first and third weeks of illness. Severe pneumonia was defined as an oxygen saturation ≤ 93% at room air. Expressions of molecules related to T-cell activation and functions were analyzed by flow cytometry.
Results: The population dynamics of T cells at the first week were not different between the two groups. However, total numbers of CD4+ and CD8 + T cells tended to be lower in the severe group at the third week of illness. Expressions of Ki-67, PD-1, perforin, and granzyme B in CD4⁺ or CD8⁺ T cells were significantly higher in the severe group than in the mild group at the third week. In contrast to the mild group, the levels of their expression did not decrease in severe group.
Conclusions: Severe COVID-19 had higher degree of proliferation, activation, and cytotoxicity of T-cells at the late phase of illness without cytotoxic T-cell contraction, which might contribute to the development of severe COVID-19.

Keywords: COVID-19; contraction; cytotoxic T cell; granzyme B; perforin; severity.