Avian Pathol . Characterization of antibody response to an epitope spanning the haemagglutinin cleavage site of H7N9 subtype avian influenza virus for differentiation of infected and vaccinated chickens

[tetano]

Avian Pathol


. 2022 Mar 17;1-25.
doi: 10.1080/03079457.2022.2054308. Online ahead of print.
Characterization of antibody response to an epitope spanning the haemagglutinin cleavage site of H7N9 subtype avian influenza virus for differentiation of infected and vaccinated chickens


Zenglei Hu ¹ , Yanyan Zhang ^{1 2} , Jiao Hu ^{2 3 4} , Shunlin Hu ^{2 3 4} , Xiufan Liu ^{1 2 3 4}



Affiliations

• PMID: 35297704
• DOI: 10.1080/03079457.2022.2054308

Abstract

AbstractH7N9 subtype avian influenza virus (AIV) is endemic in poultry in China and vaccination is used as the primary strategy for disease control. However, monitoring H7N9 virus infection in vaccinated poultry by current serological tests is difficult because vaccine-induced antibodies are not readily distinguishable from those induced by field viruses. Therefore, a test that differentiates infected and vaccinated animals (DIVA) is critical for H7N9 virus monitoring. However, no DIVA test is available for H7N9 subtype AIV. In this study, the potential of an epitope (the peptide 11) spanning the haemagglutinin (HA) cleavage site as a DIVA antigen for H7N9 virus was investigated. The results showed that the H7N9 virus infection sera and post-challenge sera obtained from H7N9 vaccinated chickens reacted with the peptide 11, whereas the sera elicited by inactivated and viral-vectored H7N9 vaccines had no reactivity with this peptide. The peptide 11 was further split in two peptides at the HA cleavage site, and the truncated peptides failed to discriminate H7N9 infected and vaccinated chickens. The peptide 11 locates in a prominent surface loop in the HA protein and contains highly conserved residues in the HA cleavage site among the H7N9 subtype and different subtypes of group 1 and 2, suggesting the potential of this peptide as a broad DIVA antigen for influenza viruses. Our study highlighted that the peptide 11 is a promising DIVA antigen and serological tests based on this peptide may serve as useful tools for monitoring H7N9 virus infection in vaccinated poultry in the field.

Keywords: DIVA; H7N9 subtype AIV; HA cleavage site; antibody response; epitope; serological tests.