J Med Virol . Development of a Monoclonal Antibody-Based Antigen Capture Enzyme-Linked Immunosorbent Assay for Detection of H7N9 Subtype Avian Influenza Virus

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J Med Virol


. 2020 Jul 10.
doi: 10.1002/jmv.26292. Online ahead of print.
Development of a Monoclonal Antibody-Based Antigen Capture Enzyme-Linked Immunosorbent Assay for Detection of H7N9 Subtype Avian Influenza Virus


Fan Yang ¹ , Yixin Xiao ¹ , Fumin Liu ¹ , Hangping Yao ¹ , Nanping Wu ¹ , Haibo Wu ¹



Affiliations

• PMID: 32648948
• DOI: 10.1002/jmv.26292

Abstract

In order to establish a rapid detection method for H7N9 avian influenza virus (AIV), monoclonal antibodies (mAbs) against hemagglutinin (HA) of H7N9 were developed to establish an antigen capture enzyme-linked immunosorbent assay (AC-ELISA). AC-ELISA achieved high specificity and sensitivity, with a detection limit of 3.9 ng/ml for H7N9 HA protein (A/Zhejiang/DTID-ZJU01/2013), and 2^-2 HA unit/100 μl for live H7N9 AIV. The inter- and intra-assay coefficient of variation was less than 10%. Compared with conventional virus isolation detection, the sensitivity and specificity were 94.96% and 88.24%, respectively. AC-ELISA proved to be a rapid and practical technique for detection of H7N9 AIV. This article is protected by copyright. All rights reserved.

Keywords: Antigen capture enzyme-linked immunosorbent assay (AC-ELISA); Assay sensitivity; Avian influenza virus; H7N9; Monoclonal antibody; Virus detection.