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Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice

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  • Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice


    Microb Cell Fact. 2020 Mar 2;19(1):53. doi: 10.1186/s12934-020-01316-1. Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice.

    Lei H1, Xie B2, Gao T2, Cen Q2, Ren Y2.
    Author information

    Abstract

    BACKGROUND:

    Existing methods for preparing influenza vaccines pose the greatest challenge against highly pandemic avian influenza H7N9 outbreak in the poultry and humans. Exploring a new strategy for manufacturing and delivering a safe and effective H7N9 vaccine is needed urgently.
    RESULTS:

    An alternative approach is to develop an influenza H7N9 oral vaccine based on yeast display technology in a timely manner. Hemagglutinin (HA) of A/Anhui/1/2013 (AH-H7N9) is used as a model antigen and characterized its expression on the surface of Saccharomyces cerevisiae (S.cerevisiae) EBY 100. Mice administrated orally with S.cerevisiae EBY100/pYD5-HA produced significant titers of IgG antibody as well as significant amounts of cytokines IFN-γ and IL-4. Importantly, S.cerevisiae EBY100/pYD5-HA could provide effective immune protection against homologous A/Anhui/1/2013 (AH-H7N9) virus challenge.
    CONCLUSIONS:

    Our findings suggest that platform based on yeast surface technology provides an alternative approach to prepare a promising influenza H7N9 oral vaccine candidate that can significantly shorten the preparedness period and result in effective protection against influenza A pandemic.


    KEYWORDS:

    Influenza A pandemic; S.cerevisiae EBY100/pYD5-HA; Yeast display technology

    PMID: 32122351 DOI: 10.1186/s12934-020-01316-1
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