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Front Microbiol. 2018 Jun 25;9:1346. doi: 10.3389/fmicb.2018.01346. eCollection 2018.
Development of an Influenza Rapid Diagnostic Kit Specific for the H7 Subtype.
Iwatsuki-Horimoto K1, Shi J2, Wang X2, Sakai-Tagawa Y1, Ito M1, Murakami K3, da Silva Lopes TJ1,4, Nakaishi K3, Yamayoshi S1, Watabe S3, Chen H2, Kawaoka Y1,4,5.
Author information
Abstract
Since the spring of 2013, human infections with H7N9 viruses have been detected in China. Some of these viruses have become highly pathogenic. Highly and low pathogenic avian influenza H7N9 viruses are currently co-circulating with the seasonal influenza A viruses H3N2 and H1N1pdm09. Prompt identification and isolation of H7N9 patients is one measure to prevent the spread of H7N9 virus and help prevent a pandemic. The majority of commercially available point-of-care rapid influenza diagnostic kits can differentiate between influenza A and B viruses, but cannot distinguish between H7N9 viruses and seasonal influenza A viruses. Accordingly, we have developed a rapid diagnostic kit specific for the H7 subtype that is accessible, easy to use. Although the detection limit of this H7 kit is one-tenth lower than that of a commercially available rapid influenza A and B diagnostic kit of similar design, except for the specificity of the monoclonal antibodies used, this kit is highly specific, detecting only H7-subtype influenza viruses, including the recent highly pathogenic H7N9 viruses from humans, and does not show any non-specific reactions with other HA subtypes. This H7 kit will be of value for the early detection of H7N9-infected patients.
KEYWORDS:
H7 subtype; highly pathogenic avian influenza; influenza virus; monoclonal antibody; rapid diagnostic kit
PMID: 29988537 PMCID: PMC6026626 DOI: 10.3389/fmicb.2018.01346
Free PMC Article
Development of an Influenza Rapid Diagnostic Kit Specific for the H7 Subtype.
Iwatsuki-Horimoto K1, Shi J2, Wang X2, Sakai-Tagawa Y1, Ito M1, Murakami K3, da Silva Lopes TJ1,4, Nakaishi K3, Yamayoshi S1, Watabe S3, Chen H2, Kawaoka Y1,4,5.
Author information
Abstract
Since the spring of 2013, human infections with H7N9 viruses have been detected in China. Some of these viruses have become highly pathogenic. Highly and low pathogenic avian influenza H7N9 viruses are currently co-circulating with the seasonal influenza A viruses H3N2 and H1N1pdm09. Prompt identification and isolation of H7N9 patients is one measure to prevent the spread of H7N9 virus and help prevent a pandemic. The majority of commercially available point-of-care rapid influenza diagnostic kits can differentiate between influenza A and B viruses, but cannot distinguish between H7N9 viruses and seasonal influenza A viruses. Accordingly, we have developed a rapid diagnostic kit specific for the H7 subtype that is accessible, easy to use. Although the detection limit of this H7 kit is one-tenth lower than that of a commercially available rapid influenza A and B diagnostic kit of similar design, except for the specificity of the monoclonal antibodies used, this kit is highly specific, detecting only H7-subtype influenza viruses, including the recent highly pathogenic H7N9 viruses from humans, and does not show any non-specific reactions with other HA subtypes. This H7 kit will be of value for the early detection of H7N9-infected patients.
KEYWORDS:
H7 subtype; highly pathogenic avian influenza; influenza virus; monoclonal antibody; rapid diagnostic kit
PMID: 29988537 PMCID: PMC6026626 DOI: 10.3389/fmicb.2018.01346
Free PMC Article