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J Virol. Continuing reassortment leads to the genetic diversity of Influenza H7N9 in Guangdong China

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  • J Virol. Continuing reassortment leads to the genetic diversity of Influenza H7N9 in Guangdong China

    [Source: Journal of Virology, full page: (LINK). Abstract, edited.]


    Continuing reassortment leads to the genetic diversity of Influenza H7N9 in Guangdong China

    Jing Lu 1,2, Jie Wu 1, Xianqiao Zeng 1, Dawei Guan 1, Lirong Zou 1, Lina Yi 1,2, Lijun Liang 1, Hanzhong Ni 1, Xin Zhang 1, Haojie Zhong 1,2, Xiang He 1,2, Corina Monagin 3, Jinyan Lin 1 and Changwen Ke 1#

    Author Affiliations: <SUP>1</SUP>Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China <SUP>2</SUP>Guangdong Provincial Institution of Public Health, Guangdong Provincial Center for Disease Control and Prevention, No. 160, Qunxian Road, Panyu District, Guangzhou, China <SUP>3</SUP>Metabiota Inc., One Sutter, Suite 600, San Francisco CA 94104 USA

    Published ahead of print 14 May 2014, doi: 10.1128/JVI.00630-14


    ABSTRACT

    On March 30, 2013, a novel avian influenza A H7N9 virus causing severe human respiratory infections was identified in China. Preliminary sequence analyses have shown that the virus is a reassortant of H7, N9 and H9N2 avian influenza viruses. In this study, we conducted enhanced surveillance for H7N9 virus in Guangdong, China, from April to August 2013. We isolated two H7N9 viral strains from environmental samples associated with poultry markets and one from a clinical patient. Sequence analyses showed that the Guangdong H7N9 virus isolated from April to May shared high sequence similarity with other strains from eastern China. The A/Guangdong/1/2013(H7N9) virus isolated from the Guangdong patient on the 10<SUP>th</SUP> of August 2013 was divergent from other previously sequenced H7N9 viruses and more closely related to local circulating H9N2 viruses in the NS and NP genes. Phylogenetic analyses revealed that four internal genes NS, NP, PB1 and PB2 of A/Guangdong/1/2013(H7N9) were in different clusters with previously identified H7N9 viruses in other provinces of China. The discovery here implicates that continuing reassortment led the A/Guangdong/1/2013(H7N9) viral emergence as a novel H7N9 virus in Guangdong China, and that viral adaptation to avian and human hosts must be assessed.


    IMPORTANCE

    In this study, we isolated and characterized the avian influenza A H7N9 virus in Guangdong, China, from April to August 2013. We showed that the viruses isolated from Guangdong environmental samples and chickens from April to May 2013 were highly similar to other H7N9 strains found in eastern China. The H7N9 virus isolated from the clinical patient in Guangdong in August 2013 was divergent from other previously identified H7N9 viruses, with the NS and NP genes originating from recent local H9N2 viruses circulating in the Province. This study provided direct evidence that continuing reassortment occurred and led to the emergence of a novel influenza H7N9 virus in Guangdong China. These results also shed light on how the H7N9 virus evolved which is critically important for future monitoring and tracing of viral transmission.


    FOOTNOTES

    # Corresponding author: Changwen Ke, Guangdong Provincial Center for Disease Control and Prevention, No.160, Qunxian Road, Dashi town, Panyu District, Guangzhou City, Guangdong Province, China, Phone: (86)2031051236. Fax: (86)2031051236, Email: kecw1965@yahoo.com.cn

    Copyright 2014, American Society for Microbiology. All Rights Reserved.


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