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Emerg Infect Dis. PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

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  • Emerg Infect Dis. PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

    [Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]


    Volume 20, Number 5óMay 2014 / Dispatch

    PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

    Wei Wang<SUP>1</SUP>, Zhigang Song<SUP>1</SUP>, Wencai Guan<SUP>1</SUP>, Yi Liu, Xiaonan Zhang, Lei Xu, Jianhua Li, Zhenghong Yuan, and Yunwen Hu

    Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China


    Abstract

    Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.


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