The PB2-K627E mutation attenuates H3N2 swine influenza virus in cultured cells and in mice

[tetano]

Res Vet Sci. 2017 Nov 11;117:54-56. doi: 10.1016/j.rvsc.2017.11.006. [Epub ahead of print]
The PB2-K627E mutation attenuates H3N2 swine influenza virus in cultured cells and in mice.

Gong XQ¹, Ruan BY¹, Liu XM¹, Zhang P¹, Wang XH¹, Wang Q¹, Shan TL¹, Tong W¹, Zhou YJ¹, Li GX¹, Zheng H¹, Tong GZ², Yu H³.
Author information

Abstract

PB2-627K is an important amino acid that determines the virulence of some influenza A viruses. However, it has not been experimentally investigated in the H3N2 swine influenza virus. To explore the potential role of PB2-K627E substitution in H3N2 swine influenza virus, the growth properties and pathogenicity between H3N2 swine influenza virus and its PB2-K627E mutant were compared. For the first time, our results showed that PB2-K627E mutation attenuates H3N2 swine influenza virus in mammalian cells and in mice, suggesting that PB2-627K is required for viral replication and pathogenicity of H3N2 swine influenza virus.


KEYWORDS:

PB2?627; Pathogenicity; Swine influenza virus; Virulence

PMID: 29175013 DOI: 10.1016/j.rvsc.2017.11.006