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Infection. 2017 Oct 31. doi: 10.1007/s15010-017-1091-3. [Epub ahead of print]
Whole genome sequencing identifies influenza A H3N2 transmission and offers superior resolution to classical typing methods.
Meinel DM1, Heinzinger S1, Eberle U1, Ackermann N1, Sch?nberger K1, Sing A2.
Author information
Abstract
OBJECTIVES:
Influenza with its annual epidemic waves is a major cause of morbidity and mortality worldwide. However, only little whole genome data are available regarding the molecular epidemiology promoting our understanding of viral spread in human populations.
METHODS:
We implemented a RT-PCR strategy starting from patient material to generate influenza A whole genome sequences for molecular epidemiological surveillance. Samples were obtained within the Bavarian Influenza Sentinel. The complete influenza virus genome was amplified by a one-tube multiplex RT-PCR and sequenced on an Illumina MiSeq.
RESULTS:
We report whole genomic sequences for 50 influenza A H3N2 viruses, which was the predominating virus in the season 2014/15, directly from patient specimens. The dataset included random samples from Bavaria (Germany) throughout the influenza season and samples from three suspected transmission clusters. We identified the outbreak samples based on sequence identity. Whole genome sequencing (WGS) was superior in resolution compared to analysis of single segments or partial segment analysis. Additionally, we detected manifestation of substantial amounts of viral quasispecies in several patients, carrying mutations varying from the dominant virus in each patient.
CONCLUSION:
Our rapid whole genome sequencing approach for influenza A virus shows that WGS can effectively be used to detect and understand outbreaks in large communities. Additionally, the genomic data provide in-depth details about the circulating virus within one season.
KEYWORDS:
Epidemiology; Influenza A; NGS; Next-generation sequencing; Outbreak analysis; Quasispecies; Surveillance; WGS; Whole genome sequencing
PMID: 29086356 DOI: 10.1007/s15010-017-1091-3
Whole genome sequencing identifies influenza A H3N2 transmission and offers superior resolution to classical typing methods.
Meinel DM1, Heinzinger S1, Eberle U1, Ackermann N1, Sch?nberger K1, Sing A2.
Author information
Abstract
OBJECTIVES:
Influenza with its annual epidemic waves is a major cause of morbidity and mortality worldwide. However, only little whole genome data are available regarding the molecular epidemiology promoting our understanding of viral spread in human populations.
METHODS:
We implemented a RT-PCR strategy starting from patient material to generate influenza A whole genome sequences for molecular epidemiological surveillance. Samples were obtained within the Bavarian Influenza Sentinel. The complete influenza virus genome was amplified by a one-tube multiplex RT-PCR and sequenced on an Illumina MiSeq.
RESULTS:
We report whole genomic sequences for 50 influenza A H3N2 viruses, which was the predominating virus in the season 2014/15, directly from patient specimens. The dataset included random samples from Bavaria (Germany) throughout the influenza season and samples from three suspected transmission clusters. We identified the outbreak samples based on sequence identity. Whole genome sequencing (WGS) was superior in resolution compared to analysis of single segments or partial segment analysis. Additionally, we detected manifestation of substantial amounts of viral quasispecies in several patients, carrying mutations varying from the dominant virus in each patient.
CONCLUSION:
Our rapid whole genome sequencing approach for influenza A virus shows that WGS can effectively be used to detect and understand outbreaks in large communities. Additionally, the genomic data provide in-depth details about the circulating virus within one season.
KEYWORDS:
Epidemiology; Influenza A; NGS; Next-generation sequencing; Outbreak analysis; Quasispecies; Surveillance; WGS; Whole genome sequencing
PMID: 29086356 DOI: 10.1007/s15010-017-1091-3