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BMC Res Notes. 2016 Aug 30;9(1):423. doi: 10.1186/s13104-016-2235-8.
Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus.
Agustiningsih A1, Trimarsanto H2,3, Setiawaty V4, Artika IM2,5, Muljono DH2,6.
Author information
Abstract
BACKGROUND:
Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009.
RESULTS:
An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested.
CONCLUSIONS:
The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009.
KEYWORDS:
HA gene; Influenza A/H3N2 virus; NA gene; Primer set
PMID: 27576569 PMCID: PMC5004302 DOI: 10.1186/s13104-016-2235-8
[PubMed - in process] Free full text
Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus.
Agustiningsih A1, Trimarsanto H2,3, Setiawaty V4, Artika IM2,5, Muljono DH2,6.
Author information
Abstract
BACKGROUND:
Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009.
RESULTS:
An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested.
CONCLUSIONS:
The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009.
KEYWORDS:
HA gene; Influenza A/H3N2 virus; NA gene; Primer set
PMID: 27576569 PMCID: PMC5004302 DOI: 10.1186/s13104-016-2235-8
[PubMed - in process] Free full text