Announcement

Collapse
No announcement yet.

J Mol Biol. Phylogenetic Analysis and Structural Modeling of SARS-CoV-2 Spike Protein Reveals an Evolutionary Distinct and Proteolytically-Sensitive Activation Loop

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • J Mol Biol. Phylogenetic Analysis and Structural Modeling of SARS-CoV-2 Spike Protein Reveals an Evolutionary Distinct and Proteolytically-Sensitive Activation Loop


    J Mol Biol. 2020 Apr 19. pii: S0022-2836(20)30287-4. doi: 10.1016/j.jmb.2020.04.009. [Epub ahead of print]
    Phylogenetic Analysis and Structural Modeling of SARS-CoV-2 Spike Protein Reveals an Evolutionary Distinct and Proteolytically-Sensitive Activation Loop.


    Jaimes JA1, Andr? NM1, Chappie JS2, Millet JK3, Whittaker GR4.

    Author information




    Abstract

    The 2019 novel coronavirus (2019-nCoV/SARS-CoV-2) originally arose as part of a major outbreak of respiratory disease centered on Hubei province China. It is now a global pandemic and is a major public health concern. Taxonomically SARS-CoV-2 was shown to be a Betacoronavirus (lineage B) closely related to SARS-CoV and SARS-related bat coronaviruses, and it has been reported to share a common receptor with SARS-CoV (ACE-2). Subsequently betacoronaviruses from pangolins were identified as close relatives to SARS-CoV-2. Here, we perform structural modeling of the SARS-CoV-2 spike glycoprotein. Our data provide support for the similar receptor utilization between SARS-CoV-2 and SARS-CoV, despite a relatively low amino acid similarity in the receptor binding module. Compared to SARS-CoV and all other coronaviruses in Betacoronavirus lineage B, we identify an extended structural loop containing basic amino acids at the interface of the receptor binding (S1) and fusion (S2) domains. We suggest this loop confers fusion activation and entry properties more in line with betacoronaviruses in lineage A and C, and be a key component in the evolution of SARS-CoV-2 with this structural loop affecting virus stability and transmission.
    Copyright ? 2020. Published by Elsevier Ltd.



    PMID:32320687DOI:10.1016/j.jmb.2020.04.009

Working...
X