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Nature. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

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  • Nature. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor


    Nature. 2020 Mar 30. doi: 10.1038/s41586-020-2180-5. [Epub ahead of print]
    Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.


    Lan J1, Ge J1, Yu J1, Shan S2, Zhou H3, Fan S1, Zhang Q2, Shi X2, Wang Q3, Zhang L4, Wang X5.

    Author information




    Abstract

    A novel and highly pathogenic coronavirus (SARS-CoV-2) has caused an outbreak in Wuhan city, Hubei province of China since December 2019, and soon spread nationwide and spilled over to other countries around the world1-3. To better understand the initial step of infection at an atomic level, we determined the crystal structure of the SARS-CoV-2 spike receptor-binding domain (RBD) bound to the cell receptor ACE2 at 2.45 ? resolution. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the SARS-CoV RBD, which also utilizes ACE2 as the cell receptor4. Structural analysis identified residues in the SARS-CoV-2 RBD that are critical for ACE2 binding, the majority of which either are highly conserved or share similar side chain properties with those in the SARS-CoV RBD. Such similarity in structure and sequence strongly argue for convergent evolution between the SARS-CoV-2 and SARS-CoV RBDs for improved binding to ACE2, although SARS-CoV-2 does not cluster within SARS and SARS-related coronaviruses1-3,5. The epitopes of two SARS-CoV antibodies targeting the RBD are also analysed with the SARS-CoV-2 RBD, providing insights into the future identification of cross-reactive antibodies.



    PMID:32225176DOI:10.1038/s41586-020-2180-5

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