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Methods Mol Biol. 2020;2123:69-79. doi: 10.1007/978-1-0716-0346-8_6.
Complete Genome Sequencing of Influenza A Viruses Using Next-Generation Sequencing.
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Abstract
Recently, chain termination sequencing methods have been replaced by more efficient next-generation sequencing (NGS) methods. For influenza A, NGS allows for deep sequencing to characterize virus populations, efficient complete genome sequencing, and a non-sequence-dependent method to identify viral variants. There are numerous approaches to preparing samples for NGS and subsequent data processing methods that can be applied to influenza A sequencing. This chapter provides a brief overview of the process of NGS for influenza A and some useful bioinformatics tools for developing an NGS workflow for influenza A viruses.
KEYWORDS:
Bioinformatics; Genome assembly; Influenza virus; Next-generation sequencing
PMID:32170681DOI:10.1007/978-1-0716-0346-8_6