Klin Lab Diagn. 2016;61(7):432-438. doi: 10.18821/0869-2084-2016-61-7-432-438.
[The development of immune enzyme test-systems for detecting potentially pandemic influenza viruses subtype A(H2), A(H5), A(H7) and A(H9).]

[Article in Russian]
Petrova ER1, Krivitskaya VZ1, Sorokin EV1, Tsareva TR1, Maiorova VG1, Timoshitcheva TA1, Sominina AA1.
Author information

1 The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia.


To develop diagnostic test-systems constructed according principle of "sandwich" enzyme-linked immunosorbent assay to detect antigens of influenza viruses with pandemic potential: A(H2), A(H5), A(H7), A(H9).The panels of subtype-specific monoclonal antibodies interacting with molecule of hemagglutinin of potentially pandemic viruses of influenza are obtained and characterized. The viruses of every sub-type were supplied with selected pairs monoclonal antibodies/monoclonal antibodies' conjugate with peroxidase of horse-radish, interacting most effectively with virus-immunogen and lacking non-specific activity related to influenza viruses of geterologic types. Considering established threshold values, the sensitivity of test-systems varied depending on sub-type and strain of viruses within limits of 4 - 30 ng/ml at evaluation of viral purified concentrates and 0.3 - 8 hemagglutinin units at analysis of virus-containing allantoic fluid. The specificity of immune-enzyme test-system manifested in conditions of absence of non-specific interactions with both seasonal and potentially pandemic geterologic viruses. The technique can be applied for fast identification of viruses of influenza non-typeable in conditions of routine functioning of practical laboratories.


detection of antigen; immune enzyme test-system; potentially pandemic viruses of influenza A(H2), A(H5), A(H7), A(H9); subtype-specific monoclonal antibodies

PMID: 31529925 DOI: 10.18821/0869-2084-2016-61-7-432-438