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Indian J Med Microbiol. 2019 Jan-Mar;37(1):105-108. doi: 10.4103/ijmm.IJMM_18_146.
Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India.
Gaur B1, Saha S2, Iuliano AD3, Rai SK4, Krishnan A4, Jain S2, Whitaker B5, Winchell J6, Lal RB2, Broor S1.
Author information
1 Department of Microbiology, All Institute of Medical Sciences, New Delhi, India. 2 Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India. 3 Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 4 Centre for Community Medicine, All Institute of Medical Sciences, New Delhi, India. 5 Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 6 Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Abstract
Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
KEYWORDS:
Micro-fluidic card; sensitivity; specificity; viral pathogen diagnosis
PMID: 31424019 DOI: 10.4103/ijmm.IJMM_18_146
Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India.
Gaur B1, Saha S2, Iuliano AD3, Rai SK4, Krishnan A4, Jain S2, Whitaker B5, Winchell J6, Lal RB2, Broor S1.
Author information
1 Department of Microbiology, All Institute of Medical Sciences, New Delhi, India. 2 Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India. 3 Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 4 Centre for Community Medicine, All Institute of Medical Sciences, New Delhi, India. 5 Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. 6 Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Abstract
Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
KEYWORDS:
Micro-fluidic card; sensitivity; specificity; viral pathogen diagnosis
PMID: 31424019 DOI: 10.4103/ijmm.IJMM_18_146