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Open Access Maced J Med Sci. 2019 Jun 30;7(12):1926-1930. doi: 10.3889/oamjms.2019.551. eCollection 2019 Jun 30.
Sputum Quality Assessment Regarding Sputum Culture for Diagnosing Lower Respiratory Tract Infections in Children.
Popova G1, Boskovska K2, Arnaudova-Danevska I2, Smilevska-Spasova O2, Jakovska T2.
Author information
1 Department of Clinical Microbiology, Institute for Respiratory Diseases in Children, Skopje, Republic of Macedonia. 2 Pediatric Department, Institute for Respiratory Diseases in Children, Skopje, Republic of Macedonia.
Abstract
BACKGROUND:
The clinical relevance of specimens from the lower airways is often debatable. However, they are most commonly examined for diagnosing lower respiratory tract infections (LRTIs).
AIM:
This study aimed to determine the diagnostic value of sputum quality assessment about sputum culture for diagnosing LRTIs in children.
METHODS:
In six months, a total of 1485 sputum samples were quality assessed by using Bartlett's grading system. All samples, regardless of their quality, were cultured, identified, and antimicrobial susceptibility testing was performed by Kirby-Bauer disc-diffusion method.
RESULTS:
Among the acceptable category, defined by Bartlett's grading system, 132 (63.2%) samples showed culture positivity of which Streptococcus pneumoniae 48 (36.4%) was most commonly isolated, followed by Moraxella catarrhalis 22 (16.7%) and Haemophilus influenza 21 (15.9%). Among the non-acceptable category, 185 (14.5%) samples were culture positive of which most commonly isolated were Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa with 64 (34.6%), 54 (29.2%) and 28 (15.1%), respectively.
CONCLUSION:
Sputum quality assessment is a useful tool for distinguishing the true respiratory pathogens from possible colonising flora for which antibiotic treatment should not be highly considered.
KEYWORDS:
Escherichia coli; Quality assessment; Sputum culture; Streptococcus pneumoniae
PMID: 31406530 PMCID: PMC6684435 DOI: 10.3889/oamjms.2019.551
Sputum Quality Assessment Regarding Sputum Culture for Diagnosing Lower Respiratory Tract Infections in Children.
Popova G1, Boskovska K2, Arnaudova-Danevska I2, Smilevska-Spasova O2, Jakovska T2.
Author information
1 Department of Clinical Microbiology, Institute for Respiratory Diseases in Children, Skopje, Republic of Macedonia. 2 Pediatric Department, Institute for Respiratory Diseases in Children, Skopje, Republic of Macedonia.
Abstract
BACKGROUND:
The clinical relevance of specimens from the lower airways is often debatable. However, they are most commonly examined for diagnosing lower respiratory tract infections (LRTIs).
AIM:
This study aimed to determine the diagnostic value of sputum quality assessment about sputum culture for diagnosing LRTIs in children.
METHODS:
In six months, a total of 1485 sputum samples were quality assessed by using Bartlett's grading system. All samples, regardless of their quality, were cultured, identified, and antimicrobial susceptibility testing was performed by Kirby-Bauer disc-diffusion method.
RESULTS:
Among the acceptable category, defined by Bartlett's grading system, 132 (63.2%) samples showed culture positivity of which Streptococcus pneumoniae 48 (36.4%) was most commonly isolated, followed by Moraxella catarrhalis 22 (16.7%) and Haemophilus influenza 21 (15.9%). Among the non-acceptable category, 185 (14.5%) samples were culture positive of which most commonly isolated were Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa with 64 (34.6%), 54 (29.2%) and 28 (15.1%), respectively.
CONCLUSION:
Sputum quality assessment is a useful tool for distinguishing the true respiratory pathogens from possible colonising flora for which antibiotic treatment should not be highly considered.
KEYWORDS:
Escherichia coli; Quality assessment; Sputum culture; Streptococcus pneumoniae
PMID: 31406530 PMCID: PMC6684435 DOI: 10.3889/oamjms.2019.551