Tweet
J Med Microbiol. 2019 Jul 2. doi: 10.1099/jmm.0.001032. [Epub ahead of print]
Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.
LeBlanc JJ1, ElSherif M1, Mulpuru S2, Warhuus M1, Ambrose A1, Andrew M1, Boivin G3, Bowie W4, Chit A5,6, Dos Santos G7, Green K8, Halperin SA1, Hatchette TF1, Ibarguchi B9, Johnstone J10, Katz K11, Langley JM1, Lagac?-Wiens P12, Loeb M10, Lund A1, MacKinnon-Cameron D1, McCarthy A13, McElhaney JE14, McGeer A8, Poirier A15, Powis J16, Richardson D17, Semret M18, Shinde V19, Smyth D20, Trottier S3, Valiquette L21, Webster D22, Ye L1, McNeil SA2.
Author information
Abstract
BACKGROUND:
The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.
METHODS:
Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.
RESULTS:
In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.
CONCLUSIONS:
Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.
PMID: 31264957 DOI: 10.1099/jmm.0.001032
Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.
LeBlanc JJ1, ElSherif M1, Mulpuru S2, Warhuus M1, Ambrose A1, Andrew M1, Boivin G3, Bowie W4, Chit A5,6, Dos Santos G7, Green K8, Halperin SA1, Hatchette TF1, Ibarguchi B9, Johnstone J10, Katz K11, Langley JM1, Lagac?-Wiens P12, Loeb M10, Lund A1, MacKinnon-Cameron D1, McCarthy A13, McElhaney JE14, McGeer A8, Poirier A15, Powis J16, Richardson D17, Semret M18, Shinde V19, Smyth D20, Trottier S3, Valiquette L21, Webster D22, Ye L1, McNeil SA2.
Author information
Abstract
BACKGROUND:
The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.
METHODS:
Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.
RESULTS:
In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.
CONCLUSIONS:
Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.
PMID: 31264957 DOI: 10.1099/jmm.0.001032