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Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

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  • Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

    PLoS One. 2019 May 7;14(5):e0216308. doi: 10.1371/journal.pone.0216308. eCollection 2019.
    Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.

    Maignan M1, Viglino D1, Hablot M1, Termoz Masson N1, Lebeugle A1, Collomb Muret R1, Mabiala Makele P1, Guglielmetti V1, Morand P2, Lupo J2, Forget V3, Landelle C3, Larrat S2.
    Author information

    Abstract

    STUDY OBJECTIVE:

    To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission.
    METHODS:

    This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage.
    RESULTS:

    A total of 187 patients were included over 11 days in January 2018. Median age was 70 years (interquartile range 44 to 84) and 95 (51%) were male. Nine (5%) assays had to be repeated due to failure of the first assay. The sensitivity of the rapid RT-PCR assay performed at triage was 0.98 (95% confidence interval (CI): 0.91-1.00) and the specificity was 0.99 (95% CI: 0.94-1.00). A total of 92 (49%) assays were performed at night-time or during the weekend. The median time from patient entry to rapid RT-PCR assay results was 46 [interquartile range 36-55] minutes.
    CONCLUSION:

    Rapid RT-PCR assay performed by nurses at triage to detect influenza A/B is feasible and highly accurate.


    PMID: 31063477 PMCID: PMC6504036 DOI: 10.1371/journal.pone.0216308
    Free PMC Article
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