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Macromol Biosci. 2019 Apr 18:e1800486. doi: 10.1002/mabi.201800486. [Epub ahead of print]
Surface-Independent and Oriented Immobilization of Antibody via One-Step Polydopamine/Protein G Coating: Application to Influenza Virus Immunoassay.
Moon J1,2, Byun J2, Kim H2, Jeong J3,4, Lim EK2,4, Jung J2,4, Cho S5, Cho WK5, Kang T2,4.
Author information
Abstract
For the construction of high-performance biosensor, it is important to interface bioreceptors with the sensor surface densely and in the optimal orientation. Herein, a simple surface modification method that can optimally immobilize antibodies onto various kinds of surfaces is reported. For the surface modification, a mixture of polydopamine (PDA) and protein G was employed. PDA is a representative mussel-inspired polymer, and protein G is an immunoglobulin-binding protein that enables an antibody to have an optimal orientation. The surface characteristics of PDA/Protein G mixture-coated substrates are analyzed and the PDA/protein G ratio is optimized to maximize the antibody binding efficiency. Moreover, the antibody-immobilized substrates are applied to the detection of influenza viruses with the naked eye, providing a detection limit of 2.9 ? 103 pfu mL-1 . Importantly, the several substrates (glass, SiO2 , Si, Al2 O3 , polyethylene terephthalate, polyethylene, polypropylene, and paper) can be modified by simple incubation with the mixture of PDA/protein G, and then the anti-influenza A H1N1 antibodies can be immobilized on the substrates successfully. Regardless of the substrate, the influenza viruses are detectable after the sandwich immunoreaction and silver enhancement procedure. It is anticipated that the developed PDA/protein G coating method will extend the range of applicable materials for biosensing.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KEYWORDS:
antibody immobilization; immunoassay; polydopamine; protein G; surface modification
PMID: 30997958 DOI: 10.1002/mabi.201800486
Surface-Independent and Oriented Immobilization of Antibody via One-Step Polydopamine/Protein G Coating: Application to Influenza Virus Immunoassay.
Moon J1,2, Byun J2, Kim H2, Jeong J3,4, Lim EK2,4, Jung J2,4, Cho S5, Cho WK5, Kang T2,4.
Author information
Abstract
For the construction of high-performance biosensor, it is important to interface bioreceptors with the sensor surface densely and in the optimal orientation. Herein, a simple surface modification method that can optimally immobilize antibodies onto various kinds of surfaces is reported. For the surface modification, a mixture of polydopamine (PDA) and protein G was employed. PDA is a representative mussel-inspired polymer, and protein G is an immunoglobulin-binding protein that enables an antibody to have an optimal orientation. The surface characteristics of PDA/Protein G mixture-coated substrates are analyzed and the PDA/protein G ratio is optimized to maximize the antibody binding efficiency. Moreover, the antibody-immobilized substrates are applied to the detection of influenza viruses with the naked eye, providing a detection limit of 2.9 ? 103 pfu mL-1 . Importantly, the several substrates (glass, SiO2 , Si, Al2 O3 , polyethylene terephthalate, polyethylene, polypropylene, and paper) can be modified by simple incubation with the mixture of PDA/protein G, and then the anti-influenza A H1N1 antibodies can be immobilized on the substrates successfully. Regardless of the substrate, the influenza viruses are detectable after the sandwich immunoreaction and silver enhancement procedure. It is anticipated that the developed PDA/protein G coating method will extend the range of applicable materials for biosensing.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KEYWORDS:
antibody immobilization; immunoassay; polydopamine; protein G; surface modification
PMID: 30997958 DOI: 10.1002/mabi.201800486