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Diagn Microbiol Infect Dis. 2018 Dec 4. pii: S0732-8893(18)30687-4. doi: 10.1016/j.diagmicrobio.2018.11.022. [Epub ahead of print]
Comparison of the Idylla? Respiratory (IFV-RSV) panel with the GeneXpert Xpert? Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples.
Wouters Y1, Keyaerts E2, Rector A2, Van Even E1, Vissers S3, Koletzki D4, Pattery T4, Rousseau E4, Van Ranst M2, Laffut W5.
Author information
Abstract
The objective of this study was to compare the performance of the Idylla? Respiratory (IFV-RSV) panel to the GeneXpert Xpert? Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert? assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla? assay showed excellent performance compared to the GeneXpert? assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.
Copyright ? 2018 Elsevier Inc. All rights reserved.
KEYWORDS:
GeneXpert; Idylla; Influenza; PCR; RSV; Respiratory panel
PMID: 30638655 DOI: 10.1016/j.diagmicrobio.2018.11.022
Comparison of the Idylla? Respiratory (IFV-RSV) panel with the GeneXpert Xpert? Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples.
Wouters Y1, Keyaerts E2, Rector A2, Van Even E1, Vissers S3, Koletzki D4, Pattery T4, Rousseau E4, Van Ranst M2, Laffut W5.
Author information
Abstract
The objective of this study was to compare the performance of the Idylla? Respiratory (IFV-RSV) panel to the GeneXpert Xpert? Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert? assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla? assay showed excellent performance compared to the GeneXpert? assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.
Copyright ? 2018 Elsevier Inc. All rights reserved.
KEYWORDS:
GeneXpert; Idylla; Influenza; PCR; RSV; Respiratory panel
PMID: 30638655 DOI: 10.1016/j.diagmicrobio.2018.11.022