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Theranostics. 2018 Nov 29;8(22):6132-6148. doi: 10.7150/thno.28027. eCollection 2018.
Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients.
Yeo SJ1, Kang H2, Dao TD1, Cuc BT1, Nguyen ATV1, Tien TTT1, Hang NLK3, Phuong HVM3, Thanh LT3, Mai LQ3, Rah Y4, Yu K4, Shin HJ5, Chong CK6, Choi HS2, Park H1.
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Abstract
Accurate and rapid diagnosis of highly pathogenic avian influenza A H5N1 is of critical importance for the effective clinical management of patients. Here, we developed a rapid and simultaneous detection toolkit for influenza A H5 subtype viruses in human samples based on a bioconjugate of quantum dots (QDs) assembly and a smartphone-based rapid dual fluorescent diagnostic system (SRDFDS). Methods: Two types of QDs were assembled on a latex bead to enhance the detection sensitivity and specificity of influenza A infection (QD580) and H5 subtype (QD650). The dual signals of influenza A and H5 subtype of H5N1-infected patients were detected simultaneously and quantified separately by SRDFDS equipped with two emission filters. Results: Our results showed a high sensitivity of 92.86% (13/14) and 78.57% (11/14), and a specificity of 100% (38/38, P < 0.0001) and 97.37% (37/38) for influenza A and H5 subtype detection, respectively. Conclusion: Therefore, our multiplex QD bioconjugates and SRDFDS-based influenza virus detection toolkit potentially provide accurate and meaningful diagnosis information with improved detection accuracies and sensitivities for H5N1 patients.
KEYWORDS:
H5N1; clinical patients; quantum dot; smartphone-based rapid dual fluorescent diagnostic system
PMID: 30613288 PMCID: PMC6299699 DOI: 10.7150/thno.28027
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Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients.
Yeo SJ1, Kang H2, Dao TD1, Cuc BT1, Nguyen ATV1, Tien TTT1, Hang NLK3, Phuong HVM3, Thanh LT3, Mai LQ3, Rah Y4, Yu K4, Shin HJ5, Chong CK6, Choi HS2, Park H1.
Author information
Abstract
Accurate and rapid diagnosis of highly pathogenic avian influenza A H5N1 is of critical importance for the effective clinical management of patients. Here, we developed a rapid and simultaneous detection toolkit for influenza A H5 subtype viruses in human samples based on a bioconjugate of quantum dots (QDs) assembly and a smartphone-based rapid dual fluorescent diagnostic system (SRDFDS). Methods: Two types of QDs were assembled on a latex bead to enhance the detection sensitivity and specificity of influenza A infection (QD580) and H5 subtype (QD650). The dual signals of influenza A and H5 subtype of H5N1-infected patients were detected simultaneously and quantified separately by SRDFDS equipped with two emission filters. Results: Our results showed a high sensitivity of 92.86% (13/14) and 78.57% (11/14), and a specificity of 100% (38/38, P < 0.0001) and 97.37% (37/38) for influenza A and H5 subtype detection, respectively. Conclusion: Therefore, our multiplex QD bioconjugates and SRDFDS-based influenza virus detection toolkit potentially provide accurate and meaningful diagnosis information with improved detection accuracies and sensitivities for H5N1 patients.
KEYWORDS:
H5N1; clinical patients; quantum dot; smartphone-based rapid dual fluorescent diagnostic system
PMID: 30613288 PMCID: PMC6299699 DOI: 10.7150/thno.28027
Free full text