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Jpn J Infect Dis. 2018 Oct 31. doi: 10.7883/yoken.JJID.2018.395. [Epub ahead of print]
Development and evaluation of new real-time RT-PCR assays for identifying the influenza A virus Cluster IV H3N2 variant.
Saito S1, Nakauchi M1, Takayama I1, Nagata S1, Odagiri T1, Kageyama T1.
Author information
Abstract
From 2005 to July 6, 2018, a total of 435 swine-origin influenza A H3N2 variant virus (H3N2v) human infections were reported in the USA. The largest H3N2v outbreak in the USA occurred in 2011-2012. This virus obtained the HA gene from human seasonal H3N2 influenza A viruses (seasonal H3N2) via human-to-swine transmission in the mid-1990s and was classified as Cluster IV H3N2v. For early detection of public health threats associated with Cluster IV H3N2v in distinction from seasonal H3N2, we developed highly specific and sensitive one-step real-time RT-PCR assays directly targeting the HA genes of Cluster IV H3N2v and seasonal H3N2. These assays are useful for systematic surveillance and identification of Cluster IV H3N2v.
KEYWORDS:
Cluster IV H3N2v; Detection; Influenza; Real-time RT-PCR; seasonal H3N2
PMID: 30381693 DOI: 10.7883/yoken.JJID.2018.395
Development and evaluation of new real-time RT-PCR assays for identifying the influenza A virus Cluster IV H3N2 variant.
Saito S1, Nakauchi M1, Takayama I1, Nagata S1, Odagiri T1, Kageyama T1.
Author information
Abstract
From 2005 to July 6, 2018, a total of 435 swine-origin influenza A H3N2 variant virus (H3N2v) human infections were reported in the USA. The largest H3N2v outbreak in the USA occurred in 2011-2012. This virus obtained the HA gene from human seasonal H3N2 influenza A viruses (seasonal H3N2) via human-to-swine transmission in the mid-1990s and was classified as Cluster IV H3N2v. For early detection of public health threats associated with Cluster IV H3N2v in distinction from seasonal H3N2, we developed highly specific and sensitive one-step real-time RT-PCR assays directly targeting the HA genes of Cluster IV H3N2v and seasonal H3N2. These assays are useful for systematic surveillance and identification of Cluster IV H3N2v.
KEYWORDS:
Cluster IV H3N2v; Detection; Influenza; Real-time RT-PCR; seasonal H3N2
PMID: 30381693 DOI: 10.7883/yoken.JJID.2018.395