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A newly developed tetraplex real time RT-PCR for simultaneous screening of influenza virus types A, B, C and D

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  • A newly developed tetraplex real time RT-PCR for simultaneous screening of influenza virus types A, B, C and D

    nfluenza Other Respir Viruses. 2018 Sep 28. doi: 10.1111/irv.12613. [Epub ahead of print] A newly developed tetraplex real time RT-PCR for simultaneous screening of influenza virus types A, B, C and D.

    Henritzi D1, Hoffmann B1, Wacheck S2, Pesch S2, Herrler G3, Beer M1, Harder TC1.
    Author information

    Abstract

    BACKGROUND:

    Human- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).
    OBJECTIVES:

    Development of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types.
    METHODS:

    An influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains, and more than 4,000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined.
    RESULTS:

    Two IBV, a single IDV but no ICV infections were identified by tetraplex RT-qPCR. IBV and IDV results were confirmed by conventional RT-PCR and partial sequence analysis.
    CONCLUSIONS:

    The tetraplex RT-qPCR proved fit for purpose as a sensitive, specific and high throughput tool to study influenza virus transmission at the human-animal interface. Complementing close-meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine. This article is protected by copyright. All rights reserved.
    This article is protected by copyright. All rights reserved.


    KEYWORDS:

    European surveillance; Influenza virus types A, B, C and D; multiplex RT-qPCR; swine

    PMID: 30264926 DOI: 10.1111/irv.12613
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