Announcement

Collapse
No announcement yet.

Fluorometric detection of influenza viral RNA using graphene oxide

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Fluorometric detection of influenza viral RNA using graphene oxide

    Anal Biochem. 2018 Sep 22. pii: S0003-2697(18)30892-3. doi: 10.1016/j.ab.2018.09.015. [Epub ahead of print]
    Fluorometric detection of influenza viral RNA using graphene oxide.

    Jeong S1, Kim DM1, An SY2, Kim DH3, Kim DE4.
    Author information

    Abstract

    Simple and reliable detection of influenza viruses is important for timely prescription of antiviral therapy. Here, we developed a facile fluorometric system for detection of influenza subtype viral genes using graphene oxide (GO). A fluorescent DNA probe complementary to hemagglutinin gene of influenza virus is degraded by the 5' to 3' exonuclease activity of Taq polymerase during PCR. Upon addition of GO, the released fluorophore retains fluorescence without adsorption onto GO, whereas the intact fluorescent DNA probe is adsorbed onto GO with fluorescence quenching. Our multi well plate system can detect as low as 3.8 pg of influenza viral RNA.


    KEYWORDS:

    Fluorometric diagnosis; Graphene oxide; Hemagglutinin subtype; Influenza virus

    PMID: 30253126 DOI: 10.1016/j.ab.2018.09.015
Working...
X