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Comparison of Six Sample-to-Answer Influenza A/B and RSV Nucleic Acid Amplification Assays Using Respiratory Specimens from Children

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  • Comparison of Six Sample-to-Answer Influenza A/B and RSV Nucleic Acid Amplification Assays Using Respiratory Specimens from Children

    J Clin Microbiol. 2018 Sep 5. pii: JCM.00930-18. doi: 10.1128/JCM.00930-18. [Epub ahead of print]
    Comparison of Six Sample-to-Answer Influenza A/B and RSV Nucleic Acid Amplification Assays Using Respiratory Specimens from Children.

    Banerjee D1, Kanwar N1, Hassan F1, Essmyer C2, Selvarangan R3.
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    Abstract

    Rapid and accurate detection of influenza A/B and respiratory syncytial virus (RSV) improves patient care. Sample-to-answer (STA) platforms based on nucleic acid amplification and detection of these viruses are simple, automated, and accurate. We compared six such platforms for the detection of FluA, FluB, and RSV: Cepheid GeneXpert? Xpress Flu/RSV (Xpert), Hologic Panther Fusion? Flu A/B/RSV (Fusion), Cobas? Influenza A/B & RSV (Liat), Luminex Aries? Flu A/B & RSV (Aries), Biofire FilmArray? Respiratory Panel (RP) and Diasorin Simplexa? Flu A/B & RSV (Simplexa). Nasopharyngeal (NP) swabs (n=225) from children previously tested by RP were assessed on these platforms. Results were compared to the Centers for Disease Control and Prevention (CDC)-developed real-time RT-PCR (rRT-PCR) assay for influenza A/B and RSV. Subtyping for FluA and FluB was performed for discrepant analysis where applicable. The percentage (%) sensitivities/specificities for FluA detection were 100/100 (Fusion), 98.6/99.3 (Xpert), 100/100 (Liat), 98.6/100 (Aries), 98.6/100 (Simplexa) and 100/100 (RP). Percentage (%) sensitivities/specificities for FluB detection were 100/100 (Fusion), 97.9/99.4 (Xpert), 97.9/98.3 (Liat), 93.7/99.4 (Aries), 85.4/99.4 (Simplexa) and 95.8/97.7 (RP); and for RSV detection were 98.1/99.4 (Xpert), 98.1/99.4 (Liat), 96.3/100 (Fusion), 94.4/100 (Aries), 87/94.4 (Simplexa) and 94.4/100 (RP). 75 confirmed FluA included 29 pH1N1, 39 H3N2, 4 sH1N1, and 3 untyped strains. 48 confirmed FluB included 33 strains of Yamagata, 13 of Victoria, 1 Yamagata+Victoria and 1 of unknown lineage. All six STA platforms demonstrated >95% sensitivity for FluA detection, while three platforms (Fusion, Xpert, and Liat) demonstrated >95% sensitivity for FluB and RSV.


    PMID: 30185508 DOI: 10.1128/JCM.00930-18
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