Tweet
Influenza Other Respir Viruses. 2018 Jul 18. doi: 10.1111/irv.12591. [Epub ahead of print]
Comparison of haemagglutination inhibition, Single Radial Haemolysis, virus neutralization and ELISA assays TO detect antibody levels against seasonal influenza viruses.
Trombetta CM1, Remarque EJ2, Mortier D2, Montomoli E1,3.
Author information
Abstract
BACKGROUND:
The immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques.,the most common being haemagglutination inhibition (HI), single radial haemolysis (SRH) and virus neutralization assays, which is commonly used in a Micro-Neutralization (MN) format. ELISA is not officially required, however this assay is able to measure different class-specific antibodies. The four assays identify different sets or subsets of antibodies.
OBJECTIVES:
The aim of this study was is to establish the correlation among four serological assays by using four seasonal influenza strains.
METHODS:
The HI, SRH, MN assays and ELISA were performed on four seasonal influenza strains.
RESULTS:
A strong positive correlation was found between HI and MN and between SRH and assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation is was also found between ELISA and the other "classical" assays for all the strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25mm2, a good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was is very low.
CONCLUSIONS:
There is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is a good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in for detecting antibodies against the influenza B viruses. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
ELISA ; Correlates of protection; Haemagglutination Inhibition assay; Single Radial Haemolysis; Virus Neutralization assay
PMID: 30019448 DOI: 10.1111/irv.12591
Free full text
Comparison of haemagglutination inhibition, Single Radial Haemolysis, virus neutralization and ELISA assays TO detect antibody levels against seasonal influenza viruses.
Trombetta CM1, Remarque EJ2, Mortier D2, Montomoli E1,3.
Author information
Abstract
BACKGROUND:
The immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques.,the most common being haemagglutination inhibition (HI), single radial haemolysis (SRH) and virus neutralization assays, which is commonly used in a Micro-Neutralization (MN) format. ELISA is not officially required, however this assay is able to measure different class-specific antibodies. The four assays identify different sets or subsets of antibodies.
OBJECTIVES:
The aim of this study was is to establish the correlation among four serological assays by using four seasonal influenza strains.
METHODS:
The HI, SRH, MN assays and ELISA were performed on four seasonal influenza strains.
RESULTS:
A strong positive correlation was found between HI and MN and between SRH and assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation is was also found between ELISA and the other "classical" assays for all the strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25mm2, a good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was is very low.
CONCLUSIONS:
There is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is a good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in for detecting antibodies against the influenza B viruses. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
ELISA ; Correlates of protection; Haemagglutination Inhibition assay; Single Radial Haemolysis; Virus Neutralization assay
PMID: 30019448 DOI: 10.1111/irv.12591
Free full text