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In vitro selection of electrochemical peptide probes using bioorthogonal tRNA for influenza virus detection

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  • In vitro selection of electrochemical peptide probes using bioorthogonal tRNA for influenza virus detection

    Chem Commun (Camb). 2018 May 2. doi: 10.1039/c8cc01775a. [Epub ahead of print]
    In vitro selection of electrochemical peptide probes using bioorthogonal tRNA for influenza virus detection.

    K C TB1, Tada S2, Zhu L3, Uzawa T4, Minagawa N5, Luo SC6, Zhao H7, Yu HH8, Aigaki T9, Ito Y10.
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    Abstract

    An electrosensitive peptide probe has been developed from an in vitro selection technique using biorthogonal tRNA prepared with an electroreactive non-natural amino acid, 3,4-ethylenedioxythiophene-conjugated aminophenylalanine. The selected probe quantitatively detected the influenza virus based on a signal "turn-on" mechanism. The developed strategy could be used to develop electrochemical biosensors toward a variety of targets.


    PMID: 29718049 DOI: 10.1039/c8cc01775a
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