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J Microbiol Biotechnol. 2018 Apr 12. doi: 10.4014/jmb.1801.01002. [Epub ahead of print]
Development and characterization of monoclonal antibodies against nucleoprotein for diagnosis of influenza A virus.
Phuong NH1,2, Kwak C1,2, Heo CK3, Cho EW3, Yang J1, Poo H1,2.
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Abstract
Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating anti-viral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity with those of commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3 and M34.33, exhibited higher reactivities to recombinant NP proteins and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed mAbs, M34.3 and M34.33, could be useful for the development of influenza diagnostics.
KEYWORDS:
Diagnosis; Influenza A virus; Monoclonal antibody; Nucleoprotein; Sensitivity
PMID: 29642295 DOI: 10.4014/jmb.1801.01002
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Development and characterization of monoclonal antibodies against nucleoprotein for diagnosis of influenza A virus.
Phuong NH1,2, Kwak C1,2, Heo CK3, Cho EW3, Yang J1, Poo H1,2.
Author information
Abstract
Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating anti-viral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity with those of commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3 and M34.33, exhibited higher reactivities to recombinant NP proteins and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed mAbs, M34.3 and M34.33, could be useful for the development of influenza diagnostics.
KEYWORDS:
Diagnosis; Influenza A virus; Monoclonal antibody; Nucleoprotein; Sensitivity
PMID: 29642295 DOI: 10.4014/jmb.1801.01002
Free full text