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A point-of-care diagnostic system to influenza viruses using Chip-based Ultra-Fast PCR

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  • A point-of-care diagnostic system to influenza viruses using Chip-based Ultra-Fast PCR

    J Med Virol. 2018 Feb 9. doi: 10.1002/jmv.25046. [Epub ahead of print]
    A point-of-care diagnostic system to influenza viruses using Chip-based Ultra-Fast PCR.

    Kwon SH1, Lee S1, Jang J1, Seo Y2, Lim HY3.
    Author information

    Abstract

    In order to diagnose the infectious disease from clinical samples, the various protocols such as culturing microorganism, rapid diagnostic test using chromatographic method, ELISA, conventional PCR are developed. Since a novel strain of avian influenza can be cross-infected human as well as birds and livestock due to genetic reassortment, some strains of influenza such as H7N9 and H5N1 have emerged as a severe virus which can be threaten the health of poultry as well as human. Therefore we explored the development of simultaneously and rapid diagnostic tool for seasonal influenza (A/H1N1, A/H3N2, B) and highly pathogenic avian influenza (A/H5N1, A/H7N9). We analyzed the unique nucleotide sequences of influenza types including three seasonal influenza, A/H7N9, and A/H5N1, and distinguished each type of influenza and diagnosed through One Step RT-PCR. In the results, Chip-based PCR technique can be diagnosed rapidly and directly from naked eye with EvaGreen the influenza also respiratory specimens within 23 min 15sec, including reverse transcription. The Chip-based PCR is a point-of-care system, and it is expected to reduce diagnosis time and to develop a diagnostic kit. Furthermore the Chip based PCR technique can be used for high risk pathogen in bioterror and/or biological warfare in the field. This article is protected by copyright. All rights reserved.


    KEYWORDS:

    Chip-based PCR; EvaGreen; GeneChecker; Influenza subtyping; POCT

    PMID: 29424457 DOI: 10.1002/jmv.25046

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