Tweet
Biochemistry (Mosc). 2017 Nov;82(11):1234-1248. doi: 10.1134/S0006297917110025.
The Power and Limitations of Influenza Virus Hemagglutinin Assays.
Ustinov NB1, Zavyalova EG, Smirnova IG, Kopylov AM.
Author information
Abstract
Influenza virus hemagglutinins (HAs) are surface proteins that bind to sialic acid residues at the host cell surface and ensure further virus internalization. Development of methods for the inhibition of these processes drives progress in the design of new antiviral drugs. The state of the isolated HA (i.e. combining tertiary structure and extent of oligomerization) is defined by multiple factors, like the HA source and purification method, posttranslational modifications, pH, etc. The HA state affects HA functional activity and significantly impacts the results of numerous HA assays. In this review, we analyze the power and limitations of currently used HA assays regarding the state of HA.
PMID: 29223151 DOI: 10.1134/S0006297917110025
The Power and Limitations of Influenza Virus Hemagglutinin Assays.
Ustinov NB1, Zavyalova EG, Smirnova IG, Kopylov AM.
Author information
Abstract
Influenza virus hemagglutinins (HAs) are surface proteins that bind to sialic acid residues at the host cell surface and ensure further virus internalization. Development of methods for the inhibition of these processes drives progress in the design of new antiviral drugs. The state of the isolated HA (i.e. combining tertiary structure and extent of oligomerization) is defined by multiple factors, like the HA source and purification method, posttranslational modifications, pH, etc. The HA state affects HA functional activity and significantly impacts the results of numerous HA assays. In this review, we analyze the power and limitations of currently used HA assays regarding the state of HA.
PMID: 29223151 DOI: 10.1134/S0006297917110025