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A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond

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  • A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond

    Sci Rep. 2017 Nov 16;7(1):15707. doi: 10.1038/s41598-017-15806-7.
    A rapid-response ultrasensitive biosensor for influenza virus detection using antibody modified boron-doped diamond.

    Nidzworski D1,2, Siuzdak K3, Niedziałkowski P4, Bogdanowicz R5, Sobaszek M6, Ryl J7, Weiher P1, Sawczak M3, Wnuk E4, Goddard WA 3rd8, Jaramillo-Botero A9, Ossowski T4.
    Author information

    Abstract

    According to the World Health Organization (WHO), almost 2 billion people each year are infected worldwide with flu-like pathogens including influenza. This is a contagious disease caused by viruses belonging to the family Orthomyxoviridae. Employee absenteeism caused by flu infection costs hundreds of millions of dollars every year. To successfully treat influenza virus infections, detection of the virus during the initial development phase of the infection is critical, when tens to hundreds of virus-associated molecules are present in the patient's pharynx. In this study, we describe a novel universal diamond biosensor, which enables the specific detection of the virus at ultralow concentrations, even before any clinical symptoms arise. A diamond electrode is surface-functionalized with polyclonal anti-M1 antibodies, which then serve to identify the universal biomarker for the influenza virus, M1 protein. The absorption of the M1 protein onto anti-M1 sites of the electrode change its electrochemical impedance spectra. We achieved a limit of detection of 1 fg/ml in saliva buffer for the M1 biomarker, which corresponds to 5-10 viruses per sample in 5 minutes. Furthermore, the universality of the assay was confirmed by analyzing different strains of influenza A virus.


    PMID: 29146948 DOI: 10.1038/s41598-017-15806-7
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