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Multiplex RT-PCR for Simultaneous Surveillance of Influenza A and B Viruses

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  • Multiplex RT-PCR for Simultaneous Surveillance of Influenza A and B Viruses

    J Clin Microbiol. 2017 Oct 4. pii: JCM.00957-17. doi: 10.1128/JCM.00957-17. [Epub ahead of print]
    Multiplex RT-PCR for Simultaneous Surveillance of Influenza A and B Viruses.

    Zhou B1,2, Deng YM3, Barnes JR4, Sessions O5, Chou TW1, Wilson M4, Stark TJ4, Volk M1, Spirason N6, Halpin RA2, Kamaraj US5, Ding T1, Stockwell TB2, Salvatore M7, Ghedin E1,7, Barr IG6, Wentworth DE8,4.
    Author information

    Abstract

    Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe; influenza A viruses intermittently cause pandemics. Sequence information from influenza genomes is instrumental in determining mechanisms underpinning antigenic evolution and antiviral resistance. However, due to sequence diversity and the dynamics of influenza evolution, rapid and high-throughput sequencing of influenza viruses remains a challenge. We developed a single-reaction FluA/B Multiplex RT-PCR method that amplifies the most critical genomic segments (HA, NA, and M) of seasonal influenza A and B viruses for next-generation sequencing, regardless of viral types, subtypes, or lineages. Herein we demonstrate that the strategy is highly sensitive and robust. The strategy was validated on thousands of seasonal influenza A and B virus positive specimens using multiple next-generation sequencing platforms.
    Copyright ? 2017 American Society for Microbiology.


    PMID: 28978683 DOI: 10.1128/JCM.00957-17
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