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A novel real-time RT-PCR assay for influenza C tested in Peruvian children

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  • A novel real-time RT-PCR assay for influenza C tested in Peruvian children

    J Clin Virol. 2017 Sep 1;96:12-16. doi: 10.1016/j.jcv.2017.08.014. [Epub ahead of print]
    A novel real-time RT-PCR assay for influenza C tested in Peruvian children.

    Howard LM1, Johnson M2, Gil AI3, Pekosz A4, Griffin MR5, Edwards KM1, Lanata CF6, Grijalva CG5, Williams JV7; RESPIRA-PERU Group.
    Author information

    Abstract

    BACKGROUND:

    Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.
    OBJECTIVES:

    To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.
    STUDY DESIGN:

    Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences. Primers and probes were designed based on conserved regions. Multiple primer-probe sets were tested against multiple ICV strains.
    RESULTS:

    The ICV M and NP genes offered the most conserved sequence regions. Primers and probes based on newer sequence data offered enhanced detection of ICV, especially for low titer specimens. An NP-targeted assay yielded the best performance and was capable of detecting 10-100 RNA copies per reaction. The NP assay detected multiple clinical isolates of ICV collected in a field epidemiology study conducted in Peru.
    CONCLUSIONS:

    We report a new real-time RT-PCR assay for ICV with high sensitivity and specificity.
    Copyright ? 2017 Elsevier B.V. All rights reserved.


    KEYWORDS:

    Diagnostics; Influenza C; Real-time RT-PCR

    PMID: 28917132 DOI: 10.1016/j.jcv.2017.08.014
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