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J Microbiol Biotechnol. 2017 Sep 15. doi: 10.4014/jmb.1708.08015. [Epub ahead of print]
An aptamer-based electrochemical sensor that can distinguish influenza virus subtype H1 from H5.
Lee JM1,2, Kim J1, Ryu I1, Woo HM1, Lee TG1, Jung W3, Yim S1, Jeong YJ1.
Author information
Abstract
The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we described a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscope (FE-SEM) showed that the immobilized aptamer specifically bound to only the H1 subtype but not the H5 subtype. Assessment by cyclic voltammetry (CV) also demonstrated that the immobilized aptamer on the indium thin oxide (ITO)-coated surface was specifically bound to the H1 subtype but not the H5 subtype, which was consistent with ELISA and FE-SEM. Further measurement of CV using various amount of H1 subtype provided detection limit of the immobilized aptamer, which showed that nanomolar scale of target protein was sufficient to produce the signal. These results indicated that the selected aptamer can be an effective probe for distinguishing the subtypes of influenza viruses by monitoring current changes.
KEYWORDS:
Aptamer; Cyclic voltammetry; Hemagglutinin; Influenza Virus; SELEX
PMID: 28910866 DOI: 10.4014/jmb.1708.08015
An aptamer-based electrochemical sensor that can distinguish influenza virus subtype H1 from H5.
Lee JM1,2, Kim J1, Ryu I1, Woo HM1, Lee TG1, Jung W3, Yim S1, Jeong YJ1.
Author information
Abstract
The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we described a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscope (FE-SEM) showed that the immobilized aptamer specifically bound to only the H1 subtype but not the H5 subtype. Assessment by cyclic voltammetry (CV) also demonstrated that the immobilized aptamer on the indium thin oxide (ITO)-coated surface was specifically bound to the H1 subtype but not the H5 subtype, which was consistent with ELISA and FE-SEM. Further measurement of CV using various amount of H1 subtype provided detection limit of the immobilized aptamer, which showed that nanomolar scale of target protein was sufficient to produce the signal. These results indicated that the selected aptamer can be an effective probe for distinguishing the subtypes of influenza viruses by monitoring current changes.
KEYWORDS:
Aptamer; Cyclic voltammetry; Hemagglutinin; Influenza Virus; SELEX
PMID: 28910866 DOI: 10.4014/jmb.1708.08015