Tweet
Biosens Bioelectron. 2017 Apr 2;94:677-685. doi: 10.1016/j.bios.2017.03.068. [Epub ahead of print]
Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye.
Yeo SJ1, Cuc BT1, Kim SA2, Kim DT1, Bao DT1, Tien TT1, Anh NT1, Choi DY3, Chong CK4, Kim HS5, Park H6.
Author information
Abstract
Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.
Copyright ? 2017 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza A virus; Novel pyridine-styrylpyridine fluorescent dye; Photostability; Rapid fluorescent immunochromatographic test
PMID: 28390319 DOI: 10.1016/j.bios.2017.03.068
Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye.
Yeo SJ1, Cuc BT1, Kim SA2, Kim DT1, Bao DT1, Tien TT1, Anh NT1, Choi DY3, Chong CK4, Kim HS5, Park H6.
Author information
Abstract
Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.
Copyright ? 2017 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza A virus; Novel pyridine-styrylpyridine fluorescent dye; Photostability; Rapid fluorescent immunochromatographic test
PMID: 28390319 DOI: 10.1016/j.bios.2017.03.068