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J Virol Methods. 2017 Mar 15. pii: S0166-0934(16)30521-3. doi: 10.1016/j.jviromet.2017.03.006. [Epub ahead of print]
Exploring novel sero-epidemiological tools-Effect of different storage conditions on longitudinal stability of microarray slides comprising influenza A-, measles- and Streptococcus pneumoniae antigens.
Freidl G1, Bruin E2, Schipper M3, Koopmans M4.
Author information
Abstract
In this study we evaluated the long-term stability of a microarray-based serological screening platform, containing antigens of influenza A, measles and Streptococcus pneumoniae, as part of a preparedness research program aiming to develop assays for syndromic disease detection. Spotted microarray slides were kept at four different storage regimes with varying temperature and humidity conditions. We showed that under the standard storage condition in a temperature-controlled (21?C) and desiccated environment (0% relative humidity), microarray slides remained stable for at least 22 months without loss of antigen quality, whereas the other three conditions (37?C, desiccated; Room temperature, non-desiccated; Frozen, desiccated) produced acceptable results for some antigens (influenza A, S.pneumoniae), but not for others (measles). We conclude that these arrays for multiplex antibody testing can be prepared and stored for prolonged periods of time, which aids laboratory-preparedness and facilitates sero-epidemiological studies.
Copyright ? 2017. Published by Elsevier B.V.
KEYWORDS:
Antigen; Humidity; Microarray; Serology; Stability; Storage; Temperature
PMID: 28315717 DOI: 10.1016/j.jviromet.2017.03.006
Exploring novel sero-epidemiological tools-Effect of different storage conditions on longitudinal stability of microarray slides comprising influenza A-, measles- and Streptococcus pneumoniae antigens.
Freidl G1, Bruin E2, Schipper M3, Koopmans M4.
Author information
Abstract
In this study we evaluated the long-term stability of a microarray-based serological screening platform, containing antigens of influenza A, measles and Streptococcus pneumoniae, as part of a preparedness research program aiming to develop assays for syndromic disease detection. Spotted microarray slides were kept at four different storage regimes with varying temperature and humidity conditions. We showed that under the standard storage condition in a temperature-controlled (21?C) and desiccated environment (0% relative humidity), microarray slides remained stable for at least 22 months without loss of antigen quality, whereas the other three conditions (37?C, desiccated; Room temperature, non-desiccated; Frozen, desiccated) produced acceptable results for some antigens (influenza A, S.pneumoniae), but not for others (measles). We conclude that these arrays for multiplex antibody testing can be prepared and stored for prolonged periods of time, which aids laboratory-preparedness and facilitates sero-epidemiological studies.
Copyright ? 2017. Published by Elsevier B.V.
KEYWORDS:
Antigen; Humidity; Microarray; Serology; Stability; Storage; Temperature
PMID: 28315717 DOI: 10.1016/j.jviromet.2017.03.006