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J Med Virol. 2017 Feb 18. doi: 10.1002/jmv.24796. [Epub ahead of print]
Lab-in-a-tube: Real-time molecular point-of-care diagnostics for influenza A and B using the cobas? Liat? system.
Melchers WJ1, Kuijpers J1, Sickler J2, Rahamat-Langendoen J1.
Author information
Abstract
BACKGROUND:
Rapid diagnosis of influenza A and B is important for direct treatment decisions in patient care and for the reduction of in-hospital transmissions. The new real-time PCR based molecular point-of-care (POC) assay, the cobas? Influenza A/B test on the cobas? Liat? System (cobas? Liat? Influenza A/B assay), generated a PCR result in less than 20 minutes, was evaluated for the detection of influenza A and B.
METHOD OF THE STUDY:
121 retrospectively collected respiratory specimens, previously analyzed with a routine influenza A/B test (Diagenode) were tested using the cobas? Liat? Influenza A/B assay.
RESULTS:
The cobas? Liat? Influenza A/B assay allows influenza A and B testing by RT-PCR within 20 minutes. This assay detected influenza A in 51 of 56 samples positive by the Diagenode test. The 5 discrepant results were retested with the Cepheid Influenza A/B test, confirming 2 positive cases. All 30 influenza B Diagenode positive samples were found positive by the cobas? Liat? Influenza A/B assay. Control samples (viral negative and non-influenza pathogens) were all negative by the cobas? Liat? Influenza A/B assay. The cobas? Liat? Influenza A/B assay showed a sensitivity for influenza A/B of 96% and 100%, respectively, and 100% specificity for both targets.
CONCLUSION:
The cobas? Liat? Influenza A/B assay is a useful tool for accurate, rapid, and sensitive detection of influenza A and B, offering timely and personalized patient management and infection control when implemented at the point-of-care. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza; cobas? Liat? Influenza A/B assay; diagnosis; rapid detection
PMID: 28213975 DOI: 10.1002/jmv.24796
[PubMed - as supplied by publisher]
Lab-in-a-tube: Real-time molecular point-of-care diagnostics for influenza A and B using the cobas? Liat? system.
Melchers WJ1, Kuijpers J1, Sickler J2, Rahamat-Langendoen J1.
Author information
Abstract
BACKGROUND:
Rapid diagnosis of influenza A and B is important for direct treatment decisions in patient care and for the reduction of in-hospital transmissions. The new real-time PCR based molecular point-of-care (POC) assay, the cobas? Influenza A/B test on the cobas? Liat? System (cobas? Liat? Influenza A/B assay), generated a PCR result in less than 20 minutes, was evaluated for the detection of influenza A and B.
METHOD OF THE STUDY:
121 retrospectively collected respiratory specimens, previously analyzed with a routine influenza A/B test (Diagenode) were tested using the cobas? Liat? Influenza A/B assay.
RESULTS:
The cobas? Liat? Influenza A/B assay allows influenza A and B testing by RT-PCR within 20 minutes. This assay detected influenza A in 51 of 56 samples positive by the Diagenode test. The 5 discrepant results were retested with the Cepheid Influenza A/B test, confirming 2 positive cases. All 30 influenza B Diagenode positive samples were found positive by the cobas? Liat? Influenza A/B assay. Control samples (viral negative and non-influenza pathogens) were all negative by the cobas? Liat? Influenza A/B assay. The cobas? Liat? Influenza A/B assay showed a sensitivity for influenza A/B of 96% and 100%, respectively, and 100% specificity for both targets.
CONCLUSION:
The cobas? Liat? Influenza A/B assay is a useful tool for accurate, rapid, and sensitive detection of influenza A and B, offering timely and personalized patient management and infection control when implemented at the point-of-care. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza; cobas? Liat? Influenza A/B assay; diagnosis; rapid detection
PMID: 28213975 DOI: 10.1002/jmv.24796
[PubMed - as supplied by publisher]