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Influenza Other Respir Viruses. 2017 Feb 20. doi: 10.1111/irv.12450. [Epub ahead of print]
Single Radial Haemolysis Compared to Hemagglutinin Inhibition and Microneutralization as a Correlate of Protection against Influenza A H3N2 in Children and Adolescents.
Wang B1, Russell ML2, Brewer A3, Newton J1, Singh P1, Ward BJ3, Loeb M1,4,5,6.
Author information
Abstract
BACKGROUND:
Serum antibodies are often used as correlates of protection for influenza. Three commonly used serological assays for detecting influenza-specific serum antibodies are single radial haemolysis (SRH), hemagglutinin inhibition (HAI), and microneutralization (MN). However, here are limited data on SRH as well as HAI and MN as correlates of protection against influenza in children and adolescents. There are also limited data that compare SRH to HAI and MN.
OBJECTIVES:
We sought primarily to understand how SRH titres correlate to protection against influenza infection in children and adolescents. We also compare SRH to HAI and MN.
METHODS:
732 healthy Hutterite children and adolescents aged between 3-15 years were enrolled from Saskatchewan and Alberta, Canada in the 2008-2009 flu season. Blood samples were drawn from participants at baseline and between 3 to 5 weeks post-vaccination. Serum antibodies against seasonal H3N2 influenza were measured by SRH, HAI, and MN assays.
RESULTS:
The estimates of protective efficacy fluctuated when the cut-off SRH values increased. The correlation between HAI and SRH titres was 0.53 (p <0.01); between MN and SRH 0.82 (p <0.01); between HAI and MN 0.50 (p <0.01). Sixteen percent of participants had SRH titres below the detection limit, compared to 7% and 34% for the MN and HAI assays.
CONCLUSIONS:
SRH had the worst correlation with protection against seasonal H3N2 in children and adolescents compared to MN and HAI. SRH, HAI and MN titres were significantly correlated with each other. SRH was less sensitive than MN but more sensitive than HAI. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Children and adolescents; Correlate of Protection; Hemagglutinin Inhibition; Influenza A H3N2; Microneutralization; Single Radial Haemolysis
PMID: 28218983 DOI: 10.1111/irv.12450
[PubMed - as supplied by publisher]
Single Radial Haemolysis Compared to Hemagglutinin Inhibition and Microneutralization as a Correlate of Protection against Influenza A H3N2 in Children and Adolescents.
Wang B1, Russell ML2, Brewer A3, Newton J1, Singh P1, Ward BJ3, Loeb M1,4,5,6.
Author information
Abstract
BACKGROUND:
Serum antibodies are often used as correlates of protection for influenza. Three commonly used serological assays for detecting influenza-specific serum antibodies are single radial haemolysis (SRH), hemagglutinin inhibition (HAI), and microneutralization (MN). However, here are limited data on SRH as well as HAI and MN as correlates of protection against influenza in children and adolescents. There are also limited data that compare SRH to HAI and MN.
OBJECTIVES:
We sought primarily to understand how SRH titres correlate to protection against influenza infection in children and adolescents. We also compare SRH to HAI and MN.
METHODS:
732 healthy Hutterite children and adolescents aged between 3-15 years were enrolled from Saskatchewan and Alberta, Canada in the 2008-2009 flu season. Blood samples were drawn from participants at baseline and between 3 to 5 weeks post-vaccination. Serum antibodies against seasonal H3N2 influenza were measured by SRH, HAI, and MN assays.
RESULTS:
The estimates of protective efficacy fluctuated when the cut-off SRH values increased. The correlation between HAI and SRH titres was 0.53 (p <0.01); between MN and SRH 0.82 (p <0.01); between HAI and MN 0.50 (p <0.01). Sixteen percent of participants had SRH titres below the detection limit, compared to 7% and 34% for the MN and HAI assays.
CONCLUSIONS:
SRH had the worst correlation with protection against seasonal H3N2 in children and adolescents compared to MN and HAI. SRH, HAI and MN titres were significantly correlated with each other. SRH was less sensitive than MN but more sensitive than HAI. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Children and adolescents; Correlate of Protection; Hemagglutinin Inhibition; Influenza A H3N2; Microneutralization; Single Radial Haemolysis
PMID: 28218983 DOI: 10.1111/irv.12450
[PubMed - as supplied by publisher]