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Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors

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  • Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors

    Biosens Bioelectron. 2017 Feb 16;92:234-240. doi: 10.1016/j.bios.2017.02.023. [Epub ahead of print]
    Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors.

    Horiguchi Y1, Goda T1, Matsumoto A1, Takeuchi H2, Yamaoka S2, Miyahara Y3.
    Author information

    Abstract

    A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.
    Copyright ? 2017 Elsevier B.V. All rights reserved.


    KEYWORDS:

    Biointerface; Biosensor; Influrenza virus; Label-free; Molecular recognition

    PMID: 28222368 DOI: 10.1016/j.bios.2017.02.023
    [PubMed - as supplied by publisher]
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