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J Med Virol. 2016 Dec 12. doi: 10.1002/jmv.24753. [Epub ahead of print]
Detecting and quantifying influenza virus with self- versus investigator-collected mid-turbinate nasal swabs.
Granados A1,2, Quach S1, McGeer A2,3,4, Gubbay JB1,2,5, Kwong JC1,6,7,8,9.
Author information
Abstract
We compared pairs of self- and investigator-collected mid-turbinate nasal swabs to detect and quantify influenza viral loads. We used RNase P, which reflects presence of human cells to determine adequate sample collection. Sixteen pairs of influenza-positive swabs and 25 pairs of influenza-negative swabs were included in this study. The median influenza A viral loads for self- and investigator-collected swabs were 1.68 and 1.67 log10 copies/ml, respectively (p = 0.96). RNase P loads were also similar between self- and investigator-collected swabs (p = 0.51). Self-collected mid-turbinate nasal swabs yield comparable viral loads to investigator-collected swabs, and therefore might be considered for research and clinical management. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza A; qPCR; self-collected nasal swab
PMID: 27943313 DOI: 10.1002/jmv.24753
[PubMed - as supplied by publisher]
Detecting and quantifying influenza virus with self- versus investigator-collected mid-turbinate nasal swabs.
Granados A1,2, Quach S1, McGeer A2,3,4, Gubbay JB1,2,5, Kwong JC1,6,7,8,9.
Author information
Abstract
We compared pairs of self- and investigator-collected mid-turbinate nasal swabs to detect and quantify influenza viral loads. We used RNase P, which reflects presence of human cells to determine adequate sample collection. Sixteen pairs of influenza-positive swabs and 25 pairs of influenza-negative swabs were included in this study. The median influenza A viral loads for self- and investigator-collected swabs were 1.68 and 1.67 log10 copies/ml, respectively (p = 0.96). RNase P loads were also similar between self- and investigator-collected swabs (p = 0.51). Self-collected mid-turbinate nasal swabs yield comparable viral loads to investigator-collected swabs, and therefore might be considered for research and clinical management. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza A; qPCR; self-collected nasal swab
PMID: 27943313 DOI: 10.1002/jmv.24753
[PubMed - as supplied by publisher]