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J Clin Microbiol. 2016 Oct 19. pii: JCM.01805-16. [Epub ahead of print]
Improved Detection of Respiratory Pathogens Using High-Quality Sputum with TaqMan Array Card Technology.
Wolff BJ1, Bramley AM2, Thurman KA1, Whitney CG1, Whitaker B3, Self WH4, Arnold SR5,6, Trabue C5, Wunderink RG7, McCullers J5,6,8, Edwards KM4, Jain S2, Winchell JM9.
Author information
Abstract
New diagnostic platforms often use naso- or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multi-pathogen testing to high-quality sputum specimens to determine if more pathogens could be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-quality sputum (defined as ≤10 epithelial cells/low power field [lpf] and ≥25 white blood cells/lpf or a q-score definition of 2+) were tested by TaqMan Array Card (TAC), a multi-pathogen real-time polymerase chain reaction (PCR) detection platform. Among 236 patients with matched specimens, a higher proportion of sputum specimens had ≥1 pathogen detected compared with NP/OP specimens in children (93% v. 68%, P<0.0001) and adults (88% v. 61%; P<0.0001); for each pathogen targeted, crossing threshold (Ct) values were earlier in sputum. Both bacterial (361 vs. 294) and viral detections (245 vs. 140) were more common in sputum versus NP/OP specimens, respectively, in both children and adults. When available, high-quality sputum may be useful for testing in hospitalized CAP patients.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27795345 DOI: 10.1128/JCM.01805-16
[PubMed - as supplied by publisher]
Improved Detection of Respiratory Pathogens Using High-Quality Sputum with TaqMan Array Card Technology.
Wolff BJ1, Bramley AM2, Thurman KA1, Whitney CG1, Whitaker B3, Self WH4, Arnold SR5,6, Trabue C5, Wunderink RG7, McCullers J5,6,8, Edwards KM4, Jain S2, Winchell JM9.
Author information
Abstract
New diagnostic platforms often use naso- or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multi-pathogen testing to high-quality sputum specimens to determine if more pathogens could be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-quality sputum (defined as ≤10 epithelial cells/low power field [lpf] and ≥25 white blood cells/lpf or a q-score definition of 2+) were tested by TaqMan Array Card (TAC), a multi-pathogen real-time polymerase chain reaction (PCR) detection platform. Among 236 patients with matched specimens, a higher proportion of sputum specimens had ≥1 pathogen detected compared with NP/OP specimens in children (93% v. 68%, P<0.0001) and adults (88% v. 61%; P<0.0001); for each pathogen targeted, crossing threshold (Ct) values were earlier in sputum. Both bacterial (361 vs. 294) and viral detections (245 vs. 140) were more common in sputum versus NP/OP specimens, respectively, in both children and adults. When available, high-quality sputum may be useful for testing in hospitalized CAP patients.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27795345 DOI: 10.1128/JCM.01805-16
[PubMed - as supplied by publisher]